A Study of Vision Biomembrane Assembly using Photoreactive Protein Adsorbed Polypyrrole Film

  • Lim, Jeong-Ok (Medical Research Institute, Kyungpook National University School of Medicine) ;
  • Huh, Jeung-Soo (Dept. of Metallurgical Engineering, College of Eng., Kyungpook National University)
  • Published : 2000.05.31

Abstract

A protein based vision biomembrane was fabricated by adsorbing bacteriorhodopsin into electrochemically polymerized polypyrrole film substrate mainly through strong electrostatic interaction. The immobilized bacteriorhodopsin on the polypyrrole film was demonstrated by SEM and SRET. The light signal transducing function from the bacteriorhodopsin which was adsorbed into Polypyrrole film was evaluated by electroretinogram(ERG). A wave form analysis of the electroretinogram indicated that the adsorbed bacteriorhodopsin retained its activity and light signal was obtained from the protein for at least one month.

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