BMB Reports
- Volume 32 Issue 5
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- Pages.480-485
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- 1999
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- 1976-670X(eISSN)
Characterization of Thermostable Tyrosine Phenol-Lyase from an Obligatory Symbiotic Thermophile, Symbiobacterium sp. SC-1
- Lee, Seung-Goo (Microbial Conversion RU, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
- Hong, Seung-Pyo (Microbial Conversion RU, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
- Kwak, Mi-Sun (Microbial Conversion RU, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
- Esaki, Nobuyoshi (Institute for Chemical Research, Kyoto University) ;
- Sung, Moon-Hee (Microbial Conversion RU, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
- Received : 1999.05.04
- Accepted : 1999.06.15
- Published : 1999.09.30
Abstract
Tyrosine phenol-lyase of thermophilic Symbiobacterium sp. SC-1, which is obligately and symbiotically dependent on thermophilic Bacillus sp. SK-1, was purified and characterized. The enzyme is composed of four identical subunits and contains approximately 1 mol of pyridoxal 5'-phosphate (PLP) per mol subunit as a cofactor. The enzyme showed absorption maxima at 330 and 420 nm, and lost this absorption profile by treatment with phenylhydrazine. The apparent dissociation constsnt,