BMB Reports
- Volume 32 Issue 5
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- Pages.451-455
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- 1999
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- 1976-670X(eISSN)
Biosynthesis and Metabolism of Vitamin C in Suspension Cultures of Scutellaria baicalensis
- Ahn, Young-Ock (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Kwon, Suk-Yoon (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Lee, Haeng-Soon (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
- Park, Il-Hyun (Department of Biochemistry, Chungnam National University) ;
- Kwak, Sang-Soo (Plant Biochemistry Research Unit, Korea Research Institute of Bioscience and Biotechnology)
- Received : 1999.04.23
- Accepted : 1999.05.31
- Published : 1999.09.30
Abstract
The concentrations of L-ascorbic acid (AsA, ascorbate, vitamin C) and its biosynthetic and metabolically-related enzymes such as L-galactono-1,4-lactone dehydrogenase (GLDase), ascorbate peroxidase (APX), and ascorbate oxidase (ASO) were investigated in suspension cultures of Scutellaria baicalensis. Cells growing from 4 days after subculture (DAS) to 9 DAS and from 16 DAS to 19 DAS showed a diauxic growth, and then growth rapidly decreased with further culturing. The AsA content slowly increased to 19 DAS, reached a maximum at 21 DAS (ca
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