BMB Reports
- 제32권3호
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- Pages.273-278
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- 1999
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- 1976-670X(eISSN)
Cytotoxicity of Vibrio vulnificus Cytolysin on Rat Neutrophils
- Park, Kwang-Hyun (Department of Biochemistry, Chonbuk National University Medical School and Institute for Medical Sciences) ;
- Rho, In-Whan (Department of Biochemistry, Chonbuk National University Medical School and Institute for Medical Sciences) ;
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Park, Byung-Hyun
(Department of Biochemistry, Chonbuk National University Medical School and Institute for Medical Sciences) ;
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Kim, Jong-Suk
(Department of Biochemistry, Chonbuk National University Medical School and Institute for Medical Sciences) ;
- Kim, Hyung-Rho (Department of Biochemistry, Chonbuk National University Medical School and Institute for Medical Sciences)
- 투고 : 1999.01.22
- 심사 : 1999.02.20
- 발행 : 1999.05.31
초록
Cytolysin produced by Vibrio vulnificus has been known to be lethal to mice by increasing vascular permeability and neutrophil sequestration in the lung. In the present study, a cytotoxic mechanism of V. vulnificus cytolysin on the neutrophil was investigated. Cytolysin rapidly bound to neutrophils and induced cell death, as determined by the trypan blue exclusion test. V. vulnificus cytolysin caused the depletion of cellular ATP without the release of ATP or lactate dehydrogenase. Formation of transmembrane pores was evidenced by the rapid efflux of potassium and 2-deoxy-D-[