Horticultural Science & Technology (원예과학기술지)
- Volume 17 Issue 4
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- Pages.473-475
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- 1999
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- 1226-8763(pISSN)
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- 2465-8588(eISSN)
Restoration of Fertility by Suppression of Male Sterility- Induced Gene Using an Antisense Construct
웅성불임 유전자의 발현억제를 이용한 임성회복
- Park, Young-Doo (Department of Horticulture, KyungHee University) ;
- Park, Beom-Seok (Cytogenetics Division, National Agricultural Science and Technology Institute, RDA) ;
- Kim, HyunUk (Cytogenetics Division, National Agricultural Science and Technology Institute, RDA) ;
- Jin, Yong-Moon (Cytogenetics Division, National Agricultural Science and Technology Institute, RDA)
- 박영두 (경희대학교 원예학과) ;
- 박범석 (농업과학기술원 생물자원부 세포유전과) ;
- 김현욱 (농업과학기술원 생물자원부 세포유전과) ;
- 진용문 (농업과학기술원 생물자원부 세포유전과)
- Published : 1999.08.31
Abstract
This study was carried out to restore the fertility by suppression of male sterility-induced gene using an antisense construct. Tobacco (cv. Petit Havana SR1) was transformed with the binary vector containing a GBAN215-6 promoter, an antisense diphtheria toxin (DTx-A) gene (pKDA215b) and a hygromycin resistant gene. Seventy-six confirmed transgenic plants regenerated from leaf disks were designated as the
본 연구는 antisense 유전자를 이용하여 웅성불임 식물체를 유기하는 웅성불임 유기 유전자의 발현을 억제함으로써 임성을 회복하기 위하여 실시하였다. 약특이 promoter(GBAN215-6 promoter)와 antisense 방향의 diphtheria toxin(DTx-A) 유전자로 제작된 pKDA215b로 담배(cv. petit Havana SR1)를 형질전환시키고 형질전환이 확인된 76개의
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