Restorative Dentistry and Endodontics
- Volume 24 Issue 1
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- Pages.76-87
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- 1999
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- 2234-7658(pISSN)
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- 2234-7666(eISSN)
EFFECT OF ROOT-END FILLING MATERIALS ON THE ACTIVITY OF CULTURED PERIODONTAL LIGAMENT FIBROBLASTS AND OSTEOBLASTS
수종 치근단 역충전 재료가 배양된 치주인대 섬유모세포 및 뼈모세포의 활성에 미치는 영향
- Yang, Mi-Young (Department of Conservative Dentistry, College of Dentistry, Kyung-Hee University) ;
- Choi, Gi-Woon (Department of Conservative Dentistry, College of Dentistry, Kyung-Hee University) ;
- Min, Byung-Soon (Department of Conservative Dentistry, College of Dentistry, Kyung-Hee University) ;
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Park, Sang-Jin
(Department of Conservative Dentistry, College of Dentistry, Kyung-Hee University) ;
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Choi, Ho-Young
(Department of Conservative Dentistry, College of Dentistry, Kyung-Hee University)
- 양미영 (경희대학교 치과대학 치과보존학교실) ;
- 최기운 (경희대학교 치과대학 치과보존학교실) ;
- 민병순 (경희대학교 치과대학 치과보존학교실) ;
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박상진
(경희대학교 치과대학 치과보존학교실) ;
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최호영
(경희대학교 치과대학 치과보존학교실)
- Published : 1999.04.06
Abstract
The effect of retrograde root-end filling materials(IRM, Super-EBA, Vitremer, MTA) on human periodontal ligament fibroblasts and osteoblasts was observed. The cell activities were evaluated by MTT assay, protein assay and alkaline phosphatase activity examination. The results as follows ; 1. After 24hrs culture, both E1 cells & PDL fibroblast adding root-end filling materials were suppressed cell activities but after 48hrs, cell activities were recovered. 2. Cell activity was lowest in Vitremer followed by IRM, MTA, Super-EBA. 3. Cell activity depression by Vitremer was not concerned with pH changes. 4. Protein synthesis by root-end filling materials were not significant difference in Both E1 cell & PDL fibroblasts but protein synthesis were a little increased by Super-EBA. 5. Alkaline phosphatase activity was increased in E1 cell by Super-EBA & MTA but was not significant differences in E1 cell by IRM & Vitremer. Alkaline phosphatase activity was a little depressed in PDL fibroblast by Vitremer. This findings suggest that these root-end filling materials may have important roles in promotion of PDL healing and consequently may be useful for clinical application in apical surgery.
Keywords