Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Expression of GiIthead Seabream (Sparus aurata) Growth Hormone in Escherichia coli Using Alginate Lyase Gene Promoter of Pseudomonas sp.

  • Lee Jong-Hee (Department of Biotechnology and Bioengineering, and Pukyong National University) ;
  • Choi Sun-Young (Department of Biotechnology and Bioengineering, and Pukyong National University) ;
  • Lee Sang-Bong (Department of Biotechnology and Bioengineering, and Pukyong National University) ;
  • Jin Cheal-Ho (Department of Biotechnology and Bioengineering, and Pukyong National University) ;
  • Huh Sung-Hoi (Department of Oceanography, Pukyong National University) ;
  • Kong In-Soo (Department of Biotechnology and Bioengineering, and Pukyong National University)
  • Published : 1999.06.01
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Abstract

The promoter region of alginate lyase gene (aly) from Pseudomonas sp. W7 was used for the high expression of gilthead seabream (Sparus aurata) growth hormone (GH) gene in Esherichia coli. PCR product encoding the premature segment of the growth hormone. was cloned to the downstream of aly promoter. GH was overexpressed With 46 ammo acid of alginate lyase as fusion protein. GH was immunoreactive and production of GH was repressed with supplementation of $0.4\%$ glucose into culture media.

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