대한수의학회지 (Korean Journal of Veterinary Research)
- 제39권3호
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- Pages.455-462
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- 1999
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Rat의 성주기에 따른 난포의 수와 형태변화
Numerical and morphologic changes of ovarian follicles in each stage of estrus cycle in rats
- Lee, Yoi-joo (Department of Histopathology, Gyeongsang National University Hospital) ;
- Kwak, Soo-dong (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
- 투고 : 1999.01.29
- 발행 : 1999.06.22
초록
This study was designed to investigate the number of the growing and mature follicles in each stage of estrus cycle in mature rats. Eighteen mature rats(Sprague-Dawley, initially 190~230gm) were randomly alloted into 4 groups(proestrus, estrus, metestrus, and diestrus) according to estrus cycles. The uteri and ovaries of rats were collected and then alternative sections of paraffin embedding ovaries were stained with H-E. Numbers of large, middle and small follicles or only large and middle follicles from secondary and tertiary follicles were investigated by LM photography of preparations. Small follicles were defined as secondary follicles with 2~5 cell layers of granulosa cells surrounding the oocyte, and middle follicles were defined as secondary follicles with more than 5 cell layers or with early signs of antral cavity or with more than one small cleft on either side of the oocytes and large follicles were defined as tertiary follicles with a single medium or large antral cavity. The number of follicles in a pair ovary per rat was appeared to be ranged from 207 to 370 and the mean number of these follicles was