Physico-chemical Properties and Cold Storage of River Puffer (Takifugu obscurus) Milt

황복(Takifugu obscurus) 정액의 물리$\cdot$화학적 성상과 냉장보존

To obtain the basic data for the preservation of river puffer (Takifugu obscurus) sperm, experiments were carried out on the physico-chemical properties and cold storage of milt. The average number of sperm and spermatocrit in milt stripped were $1.13\pm0.34\times10^{10}/ml$ and 64.8$\pm$1.4, respectively. Osmolality of seminal fluid was 266$\pm$2 mOsm/kg, Total protein and total lipid from sperm were higer than that from seminal fluid. $Ca^{2+}$ and $Na^{+}$ concentrations were higher in the seminal fluid than in the sperm, while $Mg^{2+}$ and $K^{+}$ concentrations were lower in the seminal fluid. When sperm of river puffer were preserved in $0\pm0.5^{\circ}C$ with various diluents for 16 days, fertilization rate was $0\~0.7\%$. It suggested that cold storage of river puffer sperm was detrimental to sperm fertility.

황복 (Takifugu obscurus)의 정자보존을 위한 기초자료를 얻고자, 정액의 특성을 파악하고 정자의 냉장보존 조건에 관한 연구를 수행하였다. 정액의 ml당 정자수는 $1.13\pm0.34\times10^{10}/ml$이었고 spermatocrit는 64.8$\pm$1.4였으며, 정장의 삼투질농도는 266$\pm$2 mOsm/kg이었다. 총 단백질 함량과 총 지질 함량은 정장에 비해 정자에서 높았으며, glucose는 검출되지 않았다. $Ca^{2+}$과 $Na^{+}$ 농도는 정자에 비해 정장에서 높았고, $Mg^{2+}$과 $K^{+}$은 정장에 비해 정자에서 높은 값을 보였다. 황복 정자를 여러 가지 희석액을 이용하여 16 일간 $0\pm0.5^{\circ}C$에서 냉장보존 하였을 때, 수정률은 $0\~0.7\%$로 보존 효과가 저조한 것으로 나타났다.