Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)
- Volume 32 Issue 4
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- Pages.476-480
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- 1999
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- 0374-8111(pISSN)
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- 2287-8815(eISSN)
Survival Rates of Trochophores from Pearl Oyster, Pinctada fucata martensii and Pacific Oyster, Crassostrea gigas Immersed in Pour Kinds of Cryoprotectant
4종류의 동해 방지제에 침지 한 진주조개, Pinctada fucata martensii와 참굴, Crassostrea gigas 담륜자의 생존율
- CHOI Youn Hee (Department of Aquaculture, Pukyong National University) ;
- CHANG Young Jin (Department of Aquaculture, Pukyong National University)
- Published : 1999.07.01
Abstract
Experiments were carried out to evaluate the tolerance of trochophores for pearl oyster, Pinctada fucata martensii and Pacific oyster, Crassostrea gigas using different concentrations of cryoprotectants : dimethyl sulfoxide (DMSO), ethylene glycol, glycerol and 1,2-propanediol. Each cryoprotectant with different concentrations was exposed for 5, 10, 15 and 20 minutes of immersion time. Survival rates were increased with decreased concentrations of cryoprotectant and decreased immersion time, and these differed from types of cryoprotectant. Survival rates of Pacific oyster trochophores were higher in DMSO and ethylene glycol, while those of pearl oyster trochophores were higher in glycerol and 1,2-propanediol. In case of trochophores from Pacific oyster, when 0.2 M sucrose was added in each cryoprotectant the survival rates were increased significantly.
조개류 유생의 동해방지제 독성에 대한 내성을 평가하기 위해 진주조개, Pinctada fucata martensii와 참굴, Crassostrea gigas의 담륜자를 4종류의 동해방지제에 침지하여 생존율을 조사하였다. 실험에 사용된 동해방지제는 각각 0.01