Quantification of Inulo-oligosaccharides Using High pH Anion Exchange Chromatography with Pulsed Amperometric Detector (HPAEC-PAD)
Kang, Su-Il
(Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, College of Agriculture and Life Science, Seoul National University)
;
Chang, Yung-Jin
(Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, College of Agriculture and Life Science, Seoul National University)
;
Kim, Kyoung-Yun
(Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, College of Agriculture and Life Science, Seoul National University)
;
Kim, Su-Il
(Department of Agricultural Chemistry and Research Center for New Bio-Materials in Agriculture, College of Agriculture and Life Science, Seoul National University)
Inulo-oligosaccharides (IOS, $F_n$, n=2-6) were purified from enzymatic hydrolysates of water-soluble extract of Jerusalem artichoke tubers. Quantification of inulo-oligosaccharides was done using high pH anion exchange chromatography with pulsed amperometric detector (HPAEC-PAD) at the concentration range of 10-100 mg/L, which was compared with that of fructo-oligosaccharides (FOS, $GF_n$, n=1-7). Peak areas per mg IOS were higher than FOS at the same degree of polymerization (DP). Specific peak areas of IOS increased proportionally as DP increased up to six, in contrast to FOS showing no linearity.