Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Effect of Sonicated Extract of Treponema Denticola on Osteoclast Differentiation

Treponema denticola 분쇄액에 의한 파골세포 형성 효과

  • Choi, Bong-Kyu (Dept. of Oral Biology College of Dentistry, Yonsei University) ;
  • Lee, Hyun-Jung (Dept. of Oral Biology College of Dentistry, Yonsei University) ;
  • Jeong, Gook-Jin (Dept. of Oral Biology College of Dentistry, Yonsei University) ;
  • Jung, Soon-Hee (Dept. of Oral Biology College of Dentistry, Yonsei University) ;
  • Kwak, Wall-Ah (Dept. of Oral Biology College of Dentistry, Yonsei University) ;
  • Yoo, Yun-Jung (Dept. of Oral Biology College of Dentistry, Yonsei University)
  • 최봉규 (연세대학교 치과대학 구강생물학교실) ;
  • 이현정 (연세대학교 치과대학 구강생물학교실) ;
  • 정국진 (연세대학교 치과대학 구강생물학교실) ;
  • 정순희 (연세대학교 치과대학 구강생물학교실) ;
  • 곽월아 (연세대학교 치과대학 구강생물학교실) ;
  • 유윤정 (연세대학교 치과대학 구강생물학교실)
  • Published : 1999.12.31
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Abstract

Alveolar bone destruction is a character-istic of periodontal disease. Treponema denticola are found in significantly increased numbers in the sites affected with periodontal disease. In order to clarify the role of T. denticola in destruction of alveo-lar bone in periodontal disease, this study was undertaken to determine the effect of sonicated extract of T. denticola on osteo-clast differentiation in co-culture system of mouse bone marrow cells and calvaria cells. The ability of osteoclast formation was estimated by counting the number of tar-tartrate resistant acid phosphatase(TRAP) positive cells. Sonicated extract of this bacteria stimulated osteoclast formation in a dose dependent manner(p<0.05). Indomathacin, an inhibitor of prostaglandin synthesis, decreased osteoclast formation induced by sonicated extract of this bacte-bacteria(p<0.05). Extract-induced osteoclast formation was decreased, when sonicated extract of bacteria was heated(p<0.05). These findings suggest that T. denticola induces osteoclast differentiation, and protein component of this bacteria and $PGE_2$ may play an important role in this process.

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