Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Development of PCR Technology for Identification of the Restriction Fragment Length Polymorphism(RFLP) of the Immunoglobulin Allotypes in Periodontal Patients

치주질환자의 면역글로블린 이종형에 따른 제한절편장 다변화 양상에 대한 PCR 기법의 개발

  • Choi, Jeom-Il (Department of Periodontology, School of Dentistry, Pusan National University) ;
  • Kim, Sung-Jo (Department of Periodontology, School of Dentistry, Pusan National University) ;
  • Kim, In-Hoo (Department of Biochemistry, School of Medicine, Dong-A University)
  • 최점일 (부산대학교 치과병원 치주과) ;
  • 김성조 (부산대학교 치과병원 치주과) ;
  • 김인후 (동아대학교 의과대학 생화학교실)
  • Published : 1999.06.30
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Abstract

The present study has been performed to develop a PCR technology to identify human immunoglobulin(Ig) allotypes with restriction fragment length polymorphism(RFLP) using a probe. Genomic DNA were ampilified with PCR tecnology using primers from peripheral blood lymphocytes of 10 periodontal patiens, whose Ig allotypes have been pre-determined by serological tecnique using heagglutination technique. The result indicated that the RFLP patterns could successfully differentiate the Ig allotypes, which suggests that this technology can be developed as a tool useful for population genetics studies.

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