Korean Journal of Agricultural Science (농업과학연구)
- Volume 26 Issue 2
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- Pages.39-48
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- 1999
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- 2466-2402(pISSN)
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- 2466-2410(eISSN)
Analysis of Genetic Polymorphisms and Similarity Using Random Amplified Polymorphic DNAs in Cattle
RAPD기법을 이용한 축우의 유전적 다형성과 유사도 분석
- Lee, S.H. (Division of Animal Science and Resources, College of Agriculture, Chungnam National University) ;
- Seo, K.W. (Division of Animal Science and Resources, College of Agriculture, Chungnam National University) ;
- Kwon, I. (Dept. of Food Science and Technology, College of Agriculture, Chungnam National University) ;
- Sung, C.K. (Dept. of Food Science and Technology, College of Agriculture, Chungnam National University) ;
- Kim, S.K. (Dept. of Animal Science, College of Woosong Information) ;
- Sang, B.C. (Division of Animal Science and Resources, College of Agriculture, Chungnam National University)
- 이상훈 (충남대학교 농과대학 동물자원학부) ;
- 서길웅 (충남대학교 농과대학 동물자원학부) ;
- 권일 (충남대학교 농과대학 식품공학과) ;
- 성창근 (충남대학교 농과대학 식품공학과) ;
- 김선균 (우송정보대학 동물과학과) ;
- 상병찬 (충남대학교 농과대학 동물자원학부)
- Published : 1999.12.30
Abstract
This study was carried out to investigate the difference and genetic similarity at the level of molecular genetics. Genomic DNA was extracted from blood of Holstein, Korean cattle, Charolais, and hybrid between Korean cattle and charolais and RAPD(random amplified polymorphic DNAs) was analyzed by PCR(polymerase chain reaction). After genetic similarity value from different breeds are analyzed, genetic similarity was estimated by UPGMA(unweighted pair-group method using average). The results obtained from this study can be summarized as follows: 1. When genomic DNA which was extracted from different breeds was subjected to electrophoresis on 1.5% agarose gel, bigger than 12.2kb was appeared. Ratio by absorbance of
본 연구는 축우 품종의 유전적 다형성을 분석하여 분자유전수준에서 축우의 품종간 판별과 유전적 유사성을 구명하고자 Holstein종과 한우, Charolais종 및 한우 교잡종의 혈액에서 DNA를 추출하여, PCR(polymerase chain reaction) 기법에 의한 RAPD(random amplified polymorphic DNAs)분석을 실시하고, 소 품종간의 유사도 지수를 추정한 후 UPGMA(unweighted pair-group method using average)방법으로 유사도를 추정하였으며 그 결과를 요약하면 다음과 같다. 1. 축우 품종들로부터 추출된 genomic DNA를 1.5% agarose gel에서 전기영동한 결과, 12.2kb 이상의 단일밴드로 나타났으며, genomic DNA의 순도는 DNA 흡광도
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