Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

DEVELOPMENT OF BONE REGENERATING MATERIAL USING BONE MORPHOGENETIC PROTEIN(rhBMP-2) AND BIORESORBABLE POLYMER

유전자재조합 인간 골형성단백2 및 생흡수성고분자를 이용한 골형성유도체의 개발

  • Lee, Jong-Ho (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Kim, Jong-Won (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Ahn, Kang-Min (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Seoul National University) ;
  • Kim, Kack-Kyun (Dept. of Oral Microbiology & Immunology, College of Dentistry, Seoul National University) ;
  • Lee, Zang-Hee (Dept. of Oral Microbiology & Immunology, College of Dentistry, Chosun University)
  • 이종호 (서울대학교 치과대학 구강악안면외과) ;
  • 김종원 (서울대학교 치과대학 구강악안면외과) ;
  • 안강민 (서울대학교 치과대학 구강악안면외과) ;
  • 김각균 (서울대학교 치과대학 구강미생물학/면역학교실) ;
  • 이장희 (조선대학교 치과대학 구강미생물학교실)
  • Published : 1999.12.31
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Abstract

We tested the bone regenerating capacity and histologic response of bioresorbable matrix-type implant, which was made with Poly(lactide-co-glycolide)(PLGA) and bone apatite for the carrier of bone morphogenetic protein(BMP). The critical size defect of 8mm in diameter was created at the calvaria of SD rats(n=18), and repaired with polymer implant with $15{\mu}g$ of rhBMP-2(n=9) or without it(n=9). At 2 weeks, 1 months after implantation, the animals were sacrificed(3 animals at every interval and group) and histologically evaluated. The calvarial defect which was repaired with polymer with BMP healed with newly formed bone about 70% of total defect. But that without BMP showed only 0 to under 30% bony healing. Inflammatory response was absent in both group through the experimental period, but there's marked foreign body giant response though it was a little less significant in polymer with BMP group. As the polymer was resorbed, the space was infiltrated and replaced by fibrovascular tissue, not by bone. In conclusion, our formulation of bioresorbable matrix implant loaded with bone morphogenetic protein works good as a bone regenerating material. However, it is mandatory to devise our system to have better osteoinductive and osteoconductive property, and less multinucleated giant cell response.

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