The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Detection of Astrovirus Infection from Hospitalized Young Children Feces by Reverse Transcription-Polymerase Chain Reaction

중증 위장관염 소아의 대변검체에서 역전사-중합효소 연쇄반응을 이용한 Astrovirus 검출

  • Park, Hae-Kyung (Department of Microbiology, Division of Molecular Biology and College of Medicine, Ewha Womans University) ;
  • Woo, So-Youn (Department of Microbiology, Division of Molecular Biology and College of Medicine, Ewha Womans University) ;
  • Seoh, Ju-Young (Department of Microbiology, Division of Molecular Biology and College of Medicine, Ewha Womans University) ;
  • Chong, Young-Hae (Department of Microbiology, Division of Molecular Biology and College of Medicine, Ewha Womans University) ;
  • Seo, Jeong-Wan (Department of Pediatrics, Division of Molecular Biology and College of Medicine, Ewha Womans University)
  • 박혜경 (이화여자대학교 의과대학 미생물학교실) ;
  • 우소연 (이화여자대학교 의과대학 미생물학교실) ;
  • 서주영 (이화여자대학교 의과대학 미생물학교실) ;
  • 정영해 (이화여자대학교 의과대학 미생물학교실) ;
  • 서정완 (이화여자대학교 의과대학 소아과학교실)
  • Published : 1999.10.30
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Abstract

Astrovirus is frequently associated with diarrhea in children. It can not be readily isolated by cell culture, and an electronmicroscope is usually used for detection of this agent. Recently in 1995 a combined method of reverse transcription-polymerase chain reaction (RT-PCR) was designed for easier detection of astrovirus, which is based on the conserved sequence in 3'-end of genomes of the 7 known serotypes of human astrovirus. As of yet there has not been any report of astrovirus data in Korea using the RT-PCR methods. The purpose of this study was to detect astrovirus incidence, severity of symptoms, seasonal variation and co infection rate with rotavirus in Korean children inpatients with diarrhea. Fecal specimens from 61 young children hospitalized with gasteroenteritis Korea from Jan. 1996 through Mar. 1997. They were examined for astroviurs infection by RT-PCR method. Results are as follows:1. Astrovirus was detected at 9.8% (6/61) from fecal specimens of children with severe diarrhea by EIA using monoclonal antibody coated plates. 2. Astorvirus was detected at 29.5% (18/61) from fecal specimens of children with severe diarrhea by RT-PCR. 3. The age of the 18 children affected by astrovirus ranged from 2 monthes to 7 years with mean of 3.0 years. 4. Mean hospital stay of the 18 children was 6.1 days. 5. Five (27.8%) astrovirus RT-PCR positive strains were confirmed in November and in December, respectively out of 18 specimens in total. 6. Astrovirus coinfection with rotavirus type G1 was confirmed in 15/16 specimens (93.8%), and with type G2 was in 1/16 specimens (6.3%).

Keywords

References

  1. 대한미생물학회지 v.31 Polymerase chain reaction으로 설사 영유아 대변검체에서 adenovirus type 40, 41의 분리 동정 박혜경;서주영;정영해;김경희
  2. 대한미생물학회지 v.32 VP7 Genotypes of Human Rotavirus from Hospitalized Children with Severe Diarrhea by Reverse Transcription-Polymerase Chain Reaction 박혜경;우소연;서주영;정영해;서정완
  3. Lancet i Viruses and gastroenteritis in infants Appleton H;Higgins PG
  4. J Clin Pathol v.31 Astrovirus-associated gastroenteritis in children Ashley CR;Caul EO;Paver WK
  5. J Clin Microbiol v.30 Astrovirus-associated Diarrhoea among Guatemalan Ambulatory Rural Children Cruz JR;Bartlett AV;Herrmann JE;Caceres P;Blacklow NR;Cano F
  6. J Med Virol v.57 Application of Electronmicroscopy, Enzyme Immunoassay, and RT-PCR to Monitor an Outbreak of Astrovirus Type 1 in a Paediatric Bone Marrow Transplant Unit Cubitt WD;Mitchell DK;Carter MJ;Willcocks MM;Holzel H
  7. NEJM v.324 Astroviruses as cause of gastroenteritis in children Herrmann JE;Taylor DN;Echeverria P;Blacklow NR
  8. J Virol Meth v.44 Detection of human astrovirus serotype 1 by the polymerase chain reaction Jonassen TO;Kjeldsberg E;Geinde B
  9. J Virol Meth v.52 Detection of all serotypes of human astrovirus by the polymerase chain reaction Jonassen TO;Monceyron C;Lee TW;Kurtz JB;Grinde B
  10. J Virol Meth v.50 Serotyping of human astrovirus strains by immunogold staining electron microscopy Kjeldsberg E
  11. J Med Virol v.9 Astrovirus-associated epidemic gastroenteritis in Japan Konno T;Suzuki H;Ishida N;Chiba R;Mochizuki K;Tsunoda A
  12. Pediatr J Infect Dis v.11 The frequency of astrovirus as a cause of diarrhae in Baltmore children Kotloff KL;Herrmann JE;Blacklow NR;Hudson RW;Wasserman SS;Morris JG Jr.
  13. J Clin Pathol v.30 Astrovirus-associated gastroenteritis in a children's ward Kurtz JB;Lee TW;Pickering D
  14. Med Microbiol Immunol v.166 Astrovirus gastroenteritis. Age distribution of antibody Kurtz JB;Lee TW
  15. J Med Virol v.3 Astrovirus infection in volunteers Kurtz JB;Lee TW
  16. J Gen Virol v.57 Serial propagation of astrovirus in tissue culture with the aid of trypsin Lee TW;Kurtz JB
  17. Epidemiol Infect v.112 Prevalence of human astrovirus serotypes in the Oxford region 1976-1992, with evidence for two new serotypes Lee TW;Krutz JB
  18. Pediatr J Infect Dis v.9 Six years retrospective survillance of gastroenteritis viruses identified at ten electron microscopy centers in the United States and Canada Lew JF;Glass RI;Petric M;LeBaron CW;Hammond GW;Miller SE;Robinson C;Boutilier J;Riepenhoff-Talty M;Payne CM;Franklin R;Oshiro LS;Jaqua MJ
  19. Lancet ii Viruses in infantile gastroenteritis in infants Madeley CR;Cosgrove BP
  20. J Pediatr v.123 Outbreaks of astrovirus gastroenteritis in day care centers Mitchell DK;Van R;Morrow AL;Monroe SS;Glass RI;Pickering LK
  21. J Clin Microbiol v.29 Detection of astrovirus in pediatric stool samples by immunoassay and RNA probe Moe CL;Allen JR;Monroe SS;Gray H Jr.;Humphrey CD;Hermann JE;Blacklow NR;Carcamo C;Koch M;Kim KH;Glass RI
  22. J Med Virol v.33 Electron microscopic reporting of gastrointestinal viruses in the United Kingdom, 1985-1987 Monroe SS;Glass RI;Noah N;Flewett TH;Caul EO;Ashton CI;Curry A;Field AM;Madeley CR;Pead PJ
  23. J Virol v.65 Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses Monroe SS;Jiang B;Stine SE;Koopmans M;Glass RI
  24. J Pediatr Gastroenterol Nutr v.1 Clinical associations of Astrovirus in childhood Nazar H;Rice S;Waler-Smith JA
  25. Epidemiol Infect v.113 Identification of astrovirus serotypes from children treated at the hospitals for sick children, London 1981-1993 Noel J;Cubitt D
  26. J Infect Dis v.170 A Large Outbreak of Acute Gastroenteritis Associated with Astrovirus among Students and Teachers in Osaka, Japan Oishi I;Yamazaki K;Kimoto T;Minekawa Y;Utagawa E;Yamazaki S;Inouye S;Grohmann GS;Monroe SS;Stine SE;Carcamo, Tamieando;Glass RI
  27. J Infect Dis v.143 A 27 nm virus isolated during at outbreak of acute infectious non-bacterial gastroenteritis in a convalescence hospital: a possible new serotype Oshiro LS;Haley CE;Roberts RR
  28. J Clin Microbiol v.341 Annual Incidence, Serotype Distribution, and Genetic Diversty of Human Astrovirus Isolates from Hospitalized Children in Melbourne, Australia Palombo EA;Bishop RF
  29. J Hyg v.83 Stool viruses in babies in Glasgow. 3 community studies Scott TM;Madeley CR;Cosgrove BP
  30. J Clin Microbiol v.32 Astrovirus as Cause of Gastroenteritis in Japan Utagawa ET;Nishizawa S;Sekine S;Hayashi Y;Ishihara Y;Oishi I;Iwasaki I;Yamashita I;Miyamura K;Yamazaki S;Inouye S;Glass RI
  31. Epidemiol Infect v.107 A dot-blot hybrization procedure for the detection of astrovirus in stool samples Willcocks NM;Carter MJ;Silcork JG;Madeley CR