Plasmid Profile and ${\beta}$-Lactamase Type of Multidrug-Resistant Salmonella typhi Isolated from Korea, 1997

국내 분리 다제내성 장티프스균의 Plasmid 및 ${\beta}$-lactamase의 특성에 관한 연구

  • Yoo, Jung-Sik (Department of Infectious Disease, Div. of Epidemiology, National Institute of Health) ;
  • Shin, Young-Hack (Department of Infectious Disease, Div. of Epidemiology, National Institute of Health) ;
  • Oh, Kyung-Soo (Department of Infectious Disease, Div. of Epidemiology, National Institute of Health) ;
  • Lee, Jeom-Kyu (Department of Infectious Disease, Div. of Epidemiology, National Institute of Health) ;
  • Kim, Ki-Sang (Department of Infectious Disease, Div. of Epidemiology, National Institute of Health)
  • 유정식 (국립보건원 감염질환부 역학조사과) ;
  • 신영학 (국립보건원 감염질환부 역학조사과) ;
  • 오경수 (국립보건원 감염질환부 역학조사과) ;
  • 이점규 (국립보건원 감염질환부 역학조사과) ;
  • 김기상 (국립보건원 감염질환부 역학조사과)
  • Published : 1999.10.30

Abstract

Eight strains of multidrug-resistant (MDR) Salmonella typhi were isolated from Kyonggi area during January-February, 1997. They were resistant to ampicillin, amoxicillin, carbenicillin, tetracycline, chloramphenicol, trimethoprim/sulfamethoxazole, trimethoprim. Eight strains had one plasmid respectively which size was approximately M.W 220 kb and showed same restriction pattern by endonuclease HindIII. The plasmid was similar to the plasmid in size that was related to multidrug resistant S. typhi isolated from southeast Asia. It were transferred by conjugation to recipient E. coli K-12 in frequency of $2.43{\times}10^4-1.73{\times}10^{-2}$ and transconjugant showed same drug-resistant pattern with donor cells. All of 8 strains produced ${\beta}$-lactamase that was assummed to TEM-l type by isoelectric focusing and PCR.

Keywords

References

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