Journal of Korean Academy of Oral and Maxillofacial Radiology (치과방사선)
- Volume 29 Issue 2
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- Pages.435-449
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- 1999
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- 1225-049X(pISSN)
Effect of Radiation Dosage Changes on the Cell Viability and the Apoptosis Induction on Normal and Tumorigenic Cells
방사선의 선량변화가 수종의 정상세포와 종양세포주의 세포활성도와 apoptosis 유발에 미치는 영향
- Park In-Woo (Department of Oral and Maxillofacial Radiology, College of Dentistry, Kangnung National University) ;
- Lee Sam-Sun (Department of Oral and Maxillofacial Radiology & Dental Research Institute, College of Dentistry, Seoul National University) ;
- Heo Min-Suk (Department of Oral and Maxillofacial Radiology & Dental Research Institute, College of Dentistry, Seoul National University) ;
- Choi Soon-Chul (Department of Oral and Maxillofacial Radiology & Dental Research Institute, College of Dentistry, Seoul National University)
- 박인우 (강릉대학교 치과대학 구강악안면방사선학 교실) ;
- 이삼선 (서울대학교 치과대학 구강악안면방사선학교실 및 치학연구소) ;
- 허민석 (서울대학교 치과대학 구강악안면방사선학교실 및 치학연구소) ;
- 최순철 (서울대학교 치과대학 구강악안면방사선학교실 및 치학연구소)
- Published : 1999.08.01
Abstract
Purpose : The study was aimed to detect the differences in the cell viability and the apoptosis induction after irradiation on normal and tumorigenic cells. Materials and Methods : The study. that was generated for two human normal cells(RHEK, HGF-l) and two human tumor cells(KB. HT-1080). was tested using MTT assay at 1 day and 3 day after irradiation and TUNEL assay under confocal laser scanning microscope at 1 day after irradiation. Single irradiation of 0.5. 1, 2. 4. and 8Gy were applied to the cells. The two fractions of 1. 2. 4. and 8Gy were separated with a 4-hour time interval. The irradiation was done with 5.38Gy/min dose rate using Cs-137 irradiator at room temperature. Results and Conclusions : 1. In 3-day group. the cell viability of HGF-1 cell was significantly decreased at 2. 4 and 8Gy irradiation, the cell viability of KB cell was significantly decreased at 8Gy irradiation and the cell viability of HT-I080 cell was significantly decreased at 4 and 8Gy irradiation. 2. There was significant difference between RHEK and KB cell line in the cell viability of 3-day group at 8Gy irradiation. There was significant difference between RHEK and HGF-1 cell line in the cell viability of 3-day group at 4 and 8Gy irradiation. 3. There was a significantly decreased cell viability in 3-day group than those in 1-day group at 2. 4 and 8Gy on HGF-1 cell. at 4 and 8Gy on HT-I080 cell. at 8Gy on KB cell. 4. We could detect DNA fragmented cells only on KB cell. Number of apoptotic cells of KB cell was significantly increased at 4 and 8Gy irradiation. However, there was no correlation between cell viability and apoptosis. 5. On all 4 cell lines, there were no differences between single and split irradiation method in cell viability and apoptosis.