Journal of Korean Academy of Oral and Maxillofacial Radiology (치과방사선)
- Volume 29 Issue 1
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- Pages.327-339
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- 1999
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- 1225-049X(pISSN)
Experimental Study on the Radiosensitivity and Chemosensitivity of A-431 Cell Line
A-431 세포주의 방사선 및 항암제의 감수성에 관한 실험적 연구
- Hong Seong-Woo (Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bio Science, Chonbuk National University) ;
- Choi Eun-Suk (Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bio Science, Chonbuk National University) ;
- Koh Kwang-Joon (Department of Oral and Maxillofacial Radiology, School of Dentistry, and Institute of Oral Bio Science, Chonbuk National University)
- 홍성우 (전북대학교 치과대학 구강악안면방사선학교실 및 구강생체과학연구소) ;
- 최은숙 (전북대학교 치과대학 구강악안면방사선학교실 및 구강생체과학연구소) ;
- 고광준 (전북대학교 치과대학 구강악안면방사선학교실 및 구강생체과학연구소)
- Published : 1999.02.01
Abstract
Objectives: The purpose of this study was to aid in the prediction of tumor cell tolerance to radiotherapy and/or chemotherapy. Material and Methods: Human epidermoid carcinoma A-431 cell lines were irradiated by 2, 4, 6, 8, 10Gy at a dose rate of 210cGy/min using /sup 60/Co Irradiator ALDORADO 8 and then were exposed to bleomycin or cisplatin at concentration of 2㎍/㎖ for 1 hour. The viable cells were determined for each radiation dose and/or each drug at the 4th day and cell surviving curves were obtained using semiautomated MTT assay. Results: The surviving fraction after irradiation of 2Gy was 0.99, and there was not significant difference of surviving fraction in comparison with the control group on A-431 cell line(P>0.05). But there were significant differences of surviving fractions at doses of 4, 6, 8, 10Gy in comparison with the control group(P<0.05). The cytotoxicity of bleomycin or cisplatin was significantly different in comparison with the control group on A-43l cell line (P<0.05). And the cytotoxicity of cisplatin was greater than that of bleomycin on A-431 cell line (P<0.05). There were significant differences of surviving fractions after irradiation of 2, 4, 6, 8, 10Gy with bleomycin or cisplatin in comparison with each group of irradiation only on A-431 cellline(P<0.05). There were significant differences of surviving fractions between the groups of irradiation with bleomycin and cisplatin at doses of 2, 4Gy(P<0.05), but there were not significant differences of surviving fractions at doses of 6, 8, 10Gy on A-431 cell line (P>0.05).