Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 27 Issue 1
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- Pages.46-53
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- 1999
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Hydrogen Evolution through Mixed Continuous Culture of Rhodopseudomonas sphaeroides and Clostridium butyricum
Rhodopseudomonas sphaeroides와 Clostridium butyricum의 혼합배양을 통한 수소생성의 연속발효계
- Go, Young-Hyun (Department of Biological Science, Ewha Womans University) ;
- Bae, Moo (Department of Biological Science, Ewha Womans University)
- Published : 1999.02.01
Abstract
The purpose of this study was to optimize the conditions of continuous mixed culture of C.butyricum and R. spaeroides K-7, which were able to produce hydrogen using biomass-dreived substrate. To investigate the possibility of continuous culture, semi-continuous culture was carried out for 20 days. In semi-continuous culture using the reactor system, the replacement rate of fresh medium was 30% of total medium volume for the highest hydrogen evolution. In continuous culture, the optimum dilution rate was determined to be 0.05