KSBB Journal
- 제14권6호
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- Pages.672-676
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- 1999
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- 1225-7117(pISSN)
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- 2288-8268(eISSN)
산소공급이 캡슐고정화 Aspergillus niger의 구연산 생산에 미치는 영향
The Effect of Oxygen Supply on the Production of Citric Acid from Encapsulated Aspergillus niger
- Park, Joong-Kon (Department of Chemical Engineering, Kyungpook National University) ;
- Jeong, Geung-Sik (Department of Chemical Engineering, Kyungpook National University)
- 발행 : 1999.12.01
초록
캡슐고정화 Aspergillus niger를 이용하여 산소공급이 구연산의 생산에 미치는 영향을 조사하였다. 칼슘알지네이트 캡슐에 고정화된 A. niger는 배양중 성장하여 2일 후에는 균사가 캡슐막을 뚫고 나왔으며 배양 8일 후에는 균사가 캡슐전체를 뒤덮었다. 배양액중의 질소원이 충분하거나 산소가 부족한 경우 균사가 느슨해졌다. 일정한 성장용액의 양에 미생물고정화 캡슐의 양이 많이 투입될 수록 구연산의 생산량은 감소하였다. 플라스크 배양의 경우 부피산소전달계수(
Encapsulated Aspergillus niger was prepared in order to inspect the effect of oxygen supply on the production of citric acid. A. niger cells which had been immobilized in the calcium alginate capsule grew and mycellia penetrated through the capsule membrane after two days of cultivation and covered over all of the capsule after eight days. The mycellia became loose when the nitrogen source was sufficient of oxygen was deficient. The larger amount of encapsulated cells were put into a given growth medium, the smaller quantity of citric acid was produced. The increase of volumetric oxygen transfer coefficient from 1.8