미생물학회지 (Korean Journal of Microbiology)
- 제35권4호
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- Pages.277-282
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- 1999
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
우분으로부터 Bacillus subtilis CH-10의 분리 및 균주가 분비하는 Cellulase의 특성에 관한 연구
Isolation and Characterization of Bacillus subtilis CH-10 Secreting Cellulase from Cattle Manure
- 김태일 (농촌진흥청 축산기술연구소) ;
- 한정대 (농촌진흥청 축산기술연구소) ;
- 전병수 (농촌진흥청 축산기술연구소) ;
- 하상우 (농촌진흥청 축산기술연구소) ;
- 양창범 (농촌진흥청 축산기술연구소) ;
- 김민균 (서울대학교 농생대 응용생물화학부)
- Kim, Tae-Il (National Livestock Research Institute, Rural Development Administration) ;
- Han, Jung-Dae (National Livestock Research Institute, Rural Development Administration) ;
- Jeon, Byoung-Soo (National Livestock Research Institute, Rural Development Administration) ;
- Ha, Sang-Woo (National Livestock Research Institute, Rural Development Administration) ;
- Yang, Chang-Bum (National Livestock Research Institute, Rural Development Administration) ;
- Kim, Min-Kyun (Division of Applied and Chemistry, School of Agricultural Biotechnology center for Plant Molecular Genetics and Breeding Research, College of Agriculture and Life science, Seoul National University)
- 발행 : 1999.01.01
초록
우분으로부터 cellulase를 생산하는 미생물을 congo red 염색과 활성측정을 통해 선발하여 cellulase 활성이 우수한 균을 분리하였다. 분리균은 생화학적, 형태학적, 균체 지방산 조성을 근거로 Bacillus subtilis CH-10으로 동정하였다. 분리균이 분비하는 효소학적 특성 중 효소생산 조건은 초기 pH7.5 및 배양돈도 50
A bacterium producing the extracellular cellulase was isolated from cattle feces and screened as cellulase activity was excellent upon congo red straining method and activity measurements. Isolate was identified as Bacillus subtilis CH-10 on the basis of morphological and biochemical properties as well as cellular fatty acids composition. The enzyme which the isolate secretes had the optimum initial pH and temperature for its induction was 7.5 and 50