Journal of Microbiology
- 제37권4호
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- Pages.185-192
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- 1999
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- 1225-8873(pISSN)
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- 1976-3794(eISSN)
Biodegradation of Phenanthrene by Sphingomonsa sp. Strain KH3-2
- Shin, Su-Kyuong (Microbiology Lab., Division of Marine Biology, Korea Ocean Research & Development Institute) ;
- Oh, Young-Sook (Microbiology Lab., Division of Marine Biology, Korea Ocean Research & Development Institute) ;
- Kim, Sang-Jin (Microbiology Lab., Division of Marine Biology, Korea Ocean Research & Development Institute)
- 발행 : 1999.01.01
초록
A phenanthrene-degrading bacterium was isolated from an oil-spilled intertidal sediment sample and identified as Sphingomonas sp. KH3-2. The strain degraded polycyclic aromatic compounds such naphthalene, fluorene, biphenyl, and dibenzothiophene. When strain KH3-2 was cultured for 28 days at 25C, a total of 500 ppm of phenanthrene was degrated with a concomitant production of biomass and Folin-Ciocalteau reactive aromatic intermediates. Analysis of intermediates during phenanthrene degradation using high-performance liquid chromatography and gas chromatography/mass spectrometry indicated that Sphingomonas sp. KH3-2 primarily degrades phenanthrene to 1-hydroxy-2-naphthoic acid (1H2NA) and further metabolizes 1H2NA through the degradation pathway of naphthalene.