The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
권호 표지

Serotype and Nucleotide Analysis of Human Rotavirus Isolates in Korea

국내분리 유아 로타바이러스의 혈청형과 염기서열 분석

  • Cha, Kwang-Jong (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
  • Song, Jin-Ook (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
  • Cho, Hong-Chan (School of Animal Life Science, College of Animal Husbandry, Konkuk University) ;
  • Kim, Yong-Hee (School of Animal Life Science, College of Animal Husbandry, Jung-Bu University) ;
  • Yu, Dae-Yeul (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Joong-Bok (School of Veterinary Medicine, College of Animal Husbandry, Konkuk University) ;
  • Lee, Chong-Kee (Department of Microbiology School of Medicine Catholic University of Taegu-Hyosung) ;
  • Koki, Taniguchi (Fujita Health University) ;
  • Yu, Jae-Hyeun (School of Animal Life Science, College of Animal Husbandry, Konkuk University)
  • 차광종 (건국대학교 축산대학 동물생명과학부) ;
  • 송진욱 (건국대학교 축산대학 동물생명과학부) ;
  • 조홍찬 (건국대학교 축산대학 동물생명과학부) ;
  • 김용휘 (중부대학교 동물자원학과) ;
  • 유대열 (KIST생명공학연구소) ;
  • 이중복 (건국대학교 축산대학 수의학부) ;
  • 이종기 (대구효성가톨릭대학교 의과대학 미생물학교실) ;
  • 곡구효보 (일본 등전보건위생대학 의학부) ;
  • 유제현 (건국대학교 축산대학 동물생명과학부)
  • Published : 1999.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Rotaviruses belong to Reoviridae causes diarrhea in human beings as well as domestic animals. This study was conducted to see what type of human rotaviruses are distributed in Seoul and Kyung-gi province. Twenty two of 81 patients showed rotavirus positive with diagnostic kit and RNA electropherosis. We isolated all of rotaviruses from the patients. Electropherotypes of 22 isolates showed 4:2:3:2 pattern whereas those migration patterns were long type. All of those isolates belonged to group A. Twenty out of 22 isolates reacted with monoclonal antibodies specific to G1, P1A and subgroup II, whereas rest of them, A-29 and K-30 reacted with subgroup I specific monoclonal antibody. The nucleotide sequence of an isolate K-21 showed $98{\sim}100%$ and $90{\sim}96%$ homologies with those of Wa and KU strain, respectively.

Keywords