Bulletin of the Korean Chemical Society

  • 제20권11호
  • /
  • Pages.1299-1302
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  • 1999
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Investigation of Transglutaminase-Induced Peptide Cross-Linking by Matrix-Assisted Laser Desorption / Ionization Time-of-Flight Mass Spectrometry

  • 발행 : 1999.11.20
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초록

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to demonstrate cross-linking of peptides induced by transglutaminase. The presence of ε-( Υ-glutamyl)lysine isopeptide cross-link in the acid hydrolysate of the cross-linking reaction mixture was also demonstrated by MALDI-TOF-MS without prior separation. MALDI-TOF-MS quickly provided peptide mass maps after pronase digestion of the cross-linked peptide adduct, which enabled us to monitor the hydrolytic sequence. Pronase appears to preferentially hydrolyze peptide bonds distant from the cross-link before hydrolyzing peptide bonds around the cross-link. The results suggest that pronase digestion followed by MALDI-TOF-MS could be used for determination of amino acid sequence around a modification site.

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참고문헌

  1. Protein Sci. v.4 Biemann, K.
  2. Science v.284 McLafferty, F.;Fridriksson, E. K.;Horn, D. M.;Lewis, M. A.;Zubarev, M. A.
  3. Anal. Chem. v.54 Barber, M.;Bordoli, R. S.;Elliot, G. J.;Sedgwick, R. D.;Taylor, A. N.
  4. Anal. Chem. v.60 Karas, M.;Hillenkamp, F.
  5. Science v.246 Fenn, J. B.;Mann, M.;Meng, C. K.;Wong, S. F.;Whitehouse, C. M.
  6. J. Agric. Food Chem. v.45 Kim, H.-J.;Leszyk, J.;Taub, I. A.
  7. Bull. Korean Chem. Soc. v.20 Kim, H.-J.;Park, S.
  8. Science v.160 Pisano, J. J.;Finlayson, J. S.;Peyton, M. P.
  9. Protein Cross-linking. Biochemical and Molecular Aspects Friedman, M.(Ed.)
  10. Radiat. Res. v.100 Kim, H.-J.;Mee, L. K.;Adelstein, S. J.;Taub, I. A.;Carr, S. A.;Reinhold, V. N.
  11. Anal. Biochem. v.172 Porta, R.;Esposito, C.;Metafora, S.;Pucci, P.;Malorni, A.;Marino, G.
  12. Biochem. Biophys. Res. Comm. v.154 Pucci, P.;Marino, G.;Metafora, S.;Esposito, C.;Porta, R.
  13. Methods in Molecular Biology, Vol. 16: Enzymes in Molecular Biology v.16 Sweeney, P. J.;Walker, J. M.;Burrell, M. M.(Ed.)