Clinical and Experimental Reproductive Medicine
- 제26권1호
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- Pages.9-20
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- 1999
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
생쥐 모델을 이용한 배아의 할구 생검법과 할구가 생검된 배아의 배양시 공배양 효과에 관한 연구: 인간에서의 착상 전 유전진단 기술 개발을 위한 동물실험 모델의 개발
Effects of Coculture on Development of Biopsied Mouse Embryos as a Preclinical Model for Preimplantation Genetic Diagnosis of Human Embryos
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김석현
(서울대학교 의과대학 산부인과학교실) ;
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류범용
(서울대학교 의학연구원 인구의학연구소) ;
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지병철
(서울대학교 의과대학 산부인과학교실) ;
- 최성미 (서울대학교 의학연구원 인구의학연구소) ;
- 김희선 (서울대학교 의학연구원 인구의학연구소) ;
-
방명걸
(한국과학기술원 의과학연구센터) ;
- 오선경 (서울대학교 의학연구원 인구의학연구소) ;
- 서창석 (서울대학교 의과대학 산부인과학교실) ;
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최영민
(서울대학교 의과대학 산부인과학교실) ;
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김정구
(서울대학교 의과대학 산부인과학교실) ;
- 문신용 (서울대학교 의과대학 산부인과학교실) ;
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이진용
(서울대학교 의과대학 산부인과학교실) ;
- 채희동 (울산대학교 의과대학 산부인과학교실) ;
- 김정훈 (울산대학교 의과대학 산부인과학교실)
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Kim, S.H.
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
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Ryu, B.Y.
(Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
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Jee, B.C.
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Choi, S.M. (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
- Kim, H.S. (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
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Pang, M.G.
(Biomedical Research Center, Korea Advanced Institute of Science and Technology) ;
- Oh, S.K. (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University) ;
- Suh, C.S. (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
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Choi, Y.M.
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
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Kim, J.G.
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Moon, S.Y. (Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
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Lee, J.Y.
(Department of Obstetrics and Gynecology, College of Medicine, Seoul National University) ;
- Chae, H.D. (Department of Obstetrics and Gynecology, College of Medicine, Ulsan University) ;
- Kim, C.H. (Department of Obstetrics and Gynecology, College of Medicine, Ulsan University)
- 발행 : 1999.03.30
초록
The genetic defects in human gametes and embryos can cause adverse effects on overall reproductive events. Biopsy of embryos for preimplantation genetic diagnosis (PGD) offers a new possibility of having children free of the genetic disease. In addition, advanced embryo culture method may enhance the effectiveness of embryo biopsy for the practical application of PGD. This experimental study was undertaken to evaluate the effects of coculture on the development in vitro of biopsied mouse embryos as a preclinical model for PGD of human embryos. Embryos were obtained after in vitro fertilization (IVF) from F1 hybrid mice (C57BLfemale/CBAmale). Using micromanipulation, 1, 2, 3 or 4 blastomeres of 8-cell stage embryos were aspirated through a hole made in the zona pellucida by zona drilling (ZD) with acidic Tyrode's solution (ATS). After biopsy of blastomeres, embryos were cultured in vitro for 110 hours in Ham's F-10 supplemented with 0.4% BSA or cocultured on the monolayer of Vero cells in the same medium. The frequence of blastocyst formation were recorded, and the embryos beyond blastocyst stage were stained with 10% Giemsa to count the total number of nuclei in each embryo. There was no significant difference in the blastocyst formation between the zona intact control group and the zona drilling (ZD) only, or biopsied groups. The hatching rate of all the treatment groups except 4/8 group was significantly higher than that of control group. In all the treatment groups, there was a significant reduction in the mean cell number of embryos beyond blastocyst stage (
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