배란유도제가 생쥐 미성숙난자의 성숙에 미치는 영향 및 여러 배양액내에서 생쥐 2세포기의 배아 발달에 관한 연구

Influence of Ovulation Induction Medicine on the Nuclear Maturation of Mouse Immature Oocytes and Developement of Mouse 2-cell Embryo in Various Culture Media

  • 이종진 (전북대학교병원 산부인과학교실) ;
  • 양춘모 (전북대학교병원 산부인과학교실) ;
  • 문현창 (전북대학교병원 산부인과학교실) ;
  • 이호성 (전북대학교병원 산부인과학교실) ;
  • 이기숙 (전북대학교병원 산부인과학교실) ;
  • 류철희 (전북대학교병원 산부인과학교실) ;
  • 김종덕 (전북대학교병원 산부인과학교실)
  • Lee, Jong-Jin (Department of Obstetrics and Gynecology Chonbuk National University Hospital) ;
  • Yang, Chun-Mo (Department of Obstetrics and Gynecology Chonbuk National University Hospital) ;
  • Moon, Hyun-Chang (Department of Obstetrics and Gynecology Chonbuk National University Hospital) ;
  • Lee, Ho-Seong (Department of Obstetrics and Gynecology Chonbuk National University Hospital) ;
  • Lee, Ky-Sook (Department of Obstetrics and Gynecology Chonbuk National University Hospital) ;
  • Rheu, Cheul-Hee (Department of Obstetrics and Gynecology Chonbuk National University Hospital) ;
  • Kim, Jong-Duk (Department of Obstetrics and Gynecology Chonbuk National University Hospital)
  • 발행 : 1999.06.30

초록

Purpose of the present study was to find the optimal ovulation induction medicine for the maturation and development of immature oocytes and culture media for 2-cell embryos in the mouse model. ICR female mouse aged 6 to 8 weeks, were stimulated with 5 IU PMSG injection. At 47 to 50 hour post-PMSG injection, ovaries were dissected out and oocytes-cumulus complexes were punctured. The oocyte-cumulus complexes were cultured in media containing various ovulation induction medicine, CC, HMG and Metrodin for 18 hours. Female ICR mice were stimulated with 5 IU PMSG and 48 hours later were injected 5 IU of hCG, then female and male mice were mated. At 48 hour post-hCG injection, oviducts were dissected out and 2-cell embryos were flushed. The 2-cell embryos were cultured in various media, Ham's F-10 media of milli-Q water $(3^{\circ})$, Ham's F-10 media of HPLC (high performance liquid chromatography, Baxter) water, Medicult media, HTF (human tubal fluid) media for 96 hours. The results were as follows. 1. When the oocytes-cumulus complexes were cultured in $10^{-9}{\mu}g/ml{\sim}10^{-8}{\mu}g/ml$ of CC, those were suppressed in meiotic maturation $(28.2{\sim}33.7%)$. Whereas the oocytes-cumulus complexes were cultured in $10^{-7}{\mu}g/ml{\sim}10^{-4}{\mu}g/ml$, these were not effected in meiotic maturation $(54.5{\sim}72.7%)$. 2. When the oocytes-cumulus complexes were cultured in $10^{-4}{\mu}g/ml{\sim}10^{-1}{\mu}g/ml$ of Metrodin, those were suppressed in meiotic maturation $(35.7{\sim}41.5%)$. Meanwhile the oocytes-cumulus complexes were cultured in $10^{-7}{\mu}g/ml{\sim}10^{-5}{\mu}g/ml$, those were not effected in meiotic maturation $(54.2{\sim}70.3%)$. 3. When the oocytes-cumulus complexes were cultured in $10^{-5}{\mu}g/ml{\sim}10^{-4}{\mu}g/ml$ of HMG, those were suppressed in meiotic maturation $(48.2{\sim}50.4%)$. As being cultured in $10^{-7}{\mu}g/ml{\sim}10^{-6}{\mu}g/ml$, increased in meiotic maturation $(75.8{\sim}80.7%)$. 4. When the 2-cell embryos were cultured in Ham's F-10 media of milli-Q water $(3^{\circ})$, Ham's F-10 media of HPLC (high performance liquid chromatograpy, Baxter) water, Medicult media, HTF (human tubal fluid) media, developmental rates to blastocyst and hatching for 96 hour were 50.0%, 45.2%, 71.5% and 95.6%, respectively.

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