Clinical and Experimental Reproductive Medicine
- 제26권3호
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- Pages.407-417
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- 1999
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
난포세포가 생쥐 난자의 Chymotrypsin에 대한 내성에 미치는 영향
Effects of Follicle Cells on the Chymotrypsin Resistance of Mouse Oocytes
- 김성임 (이화여자대학교 의과대학 의학과 및 의과학연구소 분자생물학부) ;
- 배인하 (성신여자대학교 생물학과) ;
- 김해권 (서울여자대학교 생물학과) ;
- 김성례 (이화여자대학교 의과대학 의학과 및 의과학연구소 분자생물학부)
- Kim, Seong-Im (Department of Biology, Division of Molecular Biology, Ewha Medical Research Center, College of Medicine, Ewha Womans University) ;
- Bae, In-Ha (Department of Biology, Sungshin Women's University) ;
- Kim, Hae-Kwon (Department of Biology, Seoul Women's University) ;
- Kim, Sung-Rye (Department of Biology, Division of Molecular Biology, Ewha Medical Research Center, College of Medicine, Ewha Womans University)
- 발행 : 1999.09.30
초록
Objective: Mammalian follicle cells are the most important somatic cells which help oocytes grow, mature and ovulate and thus are believed to provide oocytes with various functional and structural components. In the present study we have examined whether cumulus or granulosa cells might playa role in establishing the plasma membrane structure of mouse oocytes during meiotic maturation. Design: In particular the differential resistances of mouse oocytes against chymotrypsin treatment were examined following culture with or without cumulus or granulosa cells, or in these cell-conditioned media. Results: When mouse denuded oocytes, freed from their surrounding cumulus cells, were cultured in vitro for