Clinical and Experimental Reproductive Medicine
- 제26권3호
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- Pages.331-338
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- 1999
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
자궁내막증 환자의 복강액내 IGF가 자궁내막 기질세포 증식에 미치는 영향
Effects of Insulin-like Growth Factor in Peritoneal Fluid of Patients with Endometriosis on the Proliferation of Endometrial Stromal Cells
- 김정구 (서울대학교 의과대학 산부인과학교실) ;
- 서창석 (서울대학교 의과대학 산부인과학교실) ;
- 김석현 (서울대학교 의과대학 산부인과학교실) ;
- 최영민 (서울대학교 의과대학 산부인과학교실) ;
- 문신용 (서울대학교 의과대학 산부인과학교실) ;
- 이진용 (서울대학교 의과대학 산부인과학교실)
- Kim, Jung-Gu (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University) ;
- Suh, Chang-Seok (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University) ;
- Kim, Seok-Hyun (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University) ;
- Choi, Young-Min (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University) ;
- Moon, Shin-Yong (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University) ;
- Lee, Jin-Yong (Department of Obstetrics & Gynecology, College of Medicine, Seoul National University)
- 발행 : 1999.09.30
초록
The purposes of this study were to evaluate the effects of insulin-like growth factor (IGF)s in peritoneal fluid (PF) from patients with and without endometriosis on the proliferation of endometrial stromal cells and to investigate the effects of type I IGF receptor antibody on the response of endometrial stromal cells to PF from patients with endometriosis. IGFs in PF from patients with endometriosis (n=14) and without endometriosis (n=10) were measured by immunoradiometric assay and PF samples were divided into low IGF-I PF group (less than 85 ng/ml) and high IGF-I PF group (more than 85 ng/ml). Endometrial stromal cells from patients without endometriosis were cultured in serum free media in the presence or absence of 1 % PF and thymidine incorporation test were used to evaluate the proliferation of endometrial stromal cells. Also cultures were incubated with type I IGF receptor monoclonal antibody (