한국연초학회지 (Journal of the Korean Society of Tobacco Science)
- 제21권1호
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- Pages.64-69
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- 1999
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- 1225-2131(pISSN)
연초(Nicotiana tabacum cv. Xanthi) 배양세포로부터 Ubiquinone-10 생산을 위한 현탁배양
초록
The effect of phytohormones, light and phosphate on in vitro production of ubiquinone 10 from the suspension cultures of Nicotiana tabacum cv. Xanthi callus was investigated. The inoculum size and cultured time in the suspension culture had to be at least over 2 % of medium volume at 15 days for the excellent growth of Xanthi callus. The growth of Xanthi callus in the suspension culture was improved by addition of NAA and 2,4-D, especially NAA 1.0mg/1 alone, at the light condition. The optimal concentration of phytohormone was 0.1 mg/l 2.4-D and 1.0 mg/l NAA for productivity of ubiquinone 10 in the suspenseion of Xanthi callus. Addition of 3mM KH