KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)
- Volume 44 Issue 3
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- Pages.253-255
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- 1999
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- 0252-9777(pISSN)
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- 2287-8432(eISSN)
Detection of Soybean Mosaic Virus Using RT-PCR
- Kim, Yul-Ho (National Crop Experiment Station, RDA) ;
- Kim, Ok-Sun (National Crop Experiment Station, RDA) ;
- Lee, Bong-Choon (National Horticultural Research Instituts, RDA) ;
- Roh, Jae-Hwan (National Crop Experiment Station, RDA) ;
- Kim, Myoung-Ki (National Crop Experiment Station, RDA) ;
- Im, Dae-Joon (National Crop Experiment Station, RDA) ;
- Hur, Il-Bong (National Crop Experiment Station, RDA) ;
- Lee, Sang-Chul (Kyungpook National University)
- Published : 1999.09.01
Abstract
Reverse transcription and polymerase chain reaction (RT-PCR) assay was used to detect SMV strains. A pair of oligonucleotide primers were designed to include the cylindrical inclusion (CI) coding region between 4,176 to 5,560 nt. Amplification from the total RNA extracted from infected plants with SMV yielded a 1,385 bp DNA fragment. RT-PCR was shown to be