한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)
- 제44권1호
- /
- Pages.26-31
- /
- 1999
- /
- 0252-9777(pISSN)
- /
- 2287-8432(eISSN)
DNA Fingerprinting of Rice Cultivars using AFLP and RAPD Markers
- Cho, Young-Chan (National Crop Experiment Station RDA) ;
- Shin, Young-Seop (National Crop Experiment Station RDA) ;
- Ahn, Sang-Nag (National Crop Experiment Station RDA) ;
- Gleen B. Gregorio (International Rice Research Institute) ;
- Kang, Kyong-Ho (National Crop Experiment Station RDA) ;
- Darshan Brar (International Rice Research Institute) ;
- Moon, Huhn-Pal (National Crop Experiment Station RDA)
- 발행 : 1999.03.01
초록
This experiment was conducted to evaluate genetic variation in 48 rice accessions (Oryza sativa L.) using AFLP and RAPD markers. For AFLP, a total of 928 bands were generated with 11 primer combinations and 327 bands (35.2%) of them were polymorphic among 48 accessions. In RAPD analyses using 22 random primers 145 bands were produced, and 121 (83.4%) were polymorphic among 48 accessions. Each accession revealed a distinct fingerprint by two DNA marker systems. Cluster analysis using AFLP-based genetic similarity tended to classify rice cultivars into different groups corresponding to their varietal types and breeding pedigrees, but not using RAPD-based genetic similarity. The AFLP marker system was more sensitive than RAPD in fingerprinting of rice cultivars with narrow genetic diversity.