미생물에 의한 폐기름 탄화수소의 분해

Microbial Degradation of Hydrocarbons in the Waste Oil

폐기름 유출지역에서 분리 동정된 미생물 Xl, X2, X3은 폐유나 그 주성분인 난분해성 물질들을 유일 탄소원으로 자랄 수 있었다. Naphthalene과 2-methyl naphthalene은 7일만에 약 80$\%$ 분해되었다. Hexane과 hexadecane은 거의 대부분 분해되며 60$\%$의 분해가 폐유에서 관찰되었다. 합성 계면활성제인 Triton X-100와 Tween 20은 세포의 성장과 분해에 오히려 저해함을 보였다. Xl, X2은 그람 음성을 X3은 그람 양성을 보이며 항생제 ampicillin에 저항성을 가진다. Xl의 30kb plasmid을 E.coli에 transform하여 유전공학적 활용 가능성을 보였다.

Sediment samples from the waste-oil spilled sites were screened for microorganisms able to degrade the components of crude oil, and 3 strains that could degrade were obtained. The isolated 3 strains (Xl, X2 and X3) metabolized naphthalene and 2-methyl naphthalene about 80$\%$ as well as hexane and hexadecane about 60~70$\%$ as a sole carbon source in 7 days. The degradation of the waste oil was about 60$\%$. The addition of synthetic surfactant, Triton-X 100 or Tween 20 slightly inhibited the growth of the populations. Xl and X2 were gram negative and X3 was gram positive. Xl and X3 showed ampicillin resistancy. Xl strain having 30kb plasmid has been selected for genetic study. The plasmid was isolated and transformed into E. coli. showing the possibility of the genetically engineered degrader.