Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 23 Issue 2
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- Pages.149-157
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- 1999
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- 1226-5284(pISSN)
Control of Sperm Penetration In Vitro by Cumulus Cells in Porcine Oocytes
돼지의 체외수정시 난구세포에 의한 정자침입의 조절
- Park, C.K. (College of Animal Resources Science, Kangwon National University) ;
- Cheong, H.T. (College of Animal Resources Science, Kangwon National University) ;
- Lee, J.H. (National Livestock Research Institute, R.D.A.) ;
- Kim, I.C. (National Livestock Research Institute, R.D.A.) ;
- Yang, B.K. (College of Animal Resources Science, Kangwon National University) ;
- Kim, C.I. (College of Animal Resources Science, Kangwon National University)
- 박춘근 (강원대학교 동물자원과학대학) ;
- 정희태 (강원대학교 동물자원과학대학) ;
- 이장희 (농촌진흥청 축산기술연구소) ;
- 김인철 (농촌진흥청 축산기술연구소) ;
- 양부근 (강원대학교 동물자원과학대학) ;
- 김정익 (강원대학교 동물자원과학대학)
- Published : 1999.06.01
Abstract
The functional role of cumulus cells on the penetration and polyspermy during in vitro fertilization in porcine was examined. The penetration rate was significantly higher (P<0.01) in oocytes with (61%) than without (25%) cumulus cells, but significant differences in polysper-my rates were not observed. When hyaluronidase was added to the fertilization medium with different concentrations, penetration rates in oocytes with cumulus cells were higher than in oocytes without cumulus cells at 0 (61% vs 34% ; P<0.05), 0.01 (56% vs 35% ; P<0.05), 0.1 (66% vs 30% ; P<0.05) and 1.0mg/
본 연구는 체외에서 돼지정자의 난자 내 침입에 있어서 난구세포의 기능적인 역할을 검토하기 위하여 실시하였다. 체외수정 시 정자침입율은 난구세포 부착 (61%) 난자가 제거 (25%) 된 난자에 비해 높았으나 (P<0.001), 다정자침입에는 영향을 미치지 않았다. 한편 체외수정시 hyaluronidase 를 0, 0.01, 0.1 및 1.0mg/