게껍질로부터 섬유가공용 Chitin.Chitosan 정제에 관한 연구

A Study on Purification of Chitin and Chitosan for Textile Finishing Agent from Crab Shell

The purpose of this study was to investigate the purification of chitin and chitosan for textile finishing agent from crab shell. Weight loss rate(removing Ca and protein), degree of deacetylation, solubility and MIC(Minimum growth inhibitory concentration) value of chitosan and molecular weight of the treated crab shell were measured. The results of this study were as follows : 1) Weight loss rate(removing Ca) of crab shell treated with HCI increased with the concentration of HCI and treatment time, but it became constant over 60 min. of treatment time. 2) Weight loss rate(removing protein) of crab shell treated with NaOH(0.5N∼2N) increased with the concentration of NaOH and treatment temperature and time, but it became constant above loot of temperature and over 200 min. of treatment time. 3) Degree of deacetylation of chitin treated with NaOH increased with the concentration of NaOH(40∼60％), but molecular weight decreased and thus MIC value increased. 4) Concentration of acetic acid should be above 0.3％ to dissolve chitosan easily. Solubility for chitosan was the highest with formic acid, and the next was acetic acid, hydrochloric acid, lactic acid and sulfuric acid in order.