Journal of Microbiology and Biotechnology

  • 제9권6호
  • /
  • Pages.854-860
  • /
  • 1999
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Purification and Characterization of Iron-Containing Superoxide Dismutase from Lentinus edodes

  • 발행 : 1999.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Superoxide dismutase (SOD) was purified to homogeneity from fruiting bodies of edible mushroom, Lentinus edodes, by ammonium sulfate precipitation, diethylaminoethyl (DEAE)-Sepharose FF ion-exchange chromatography, Sephacryl S-200 gel filtration chromatography, and preparative PAGE. The molecular weight of the purified enzyme was estimated to be approximately 54 kDa by gel filtration chromatography, and the enzyme was shown to be consisted of two identical subunits of molecular weight 27 kDa by SDS-PAGE. The isoelectric point of the enzyme was 4.9 as determined by isoelectric focusing. The enzyme had optimal pH and temperature of pH 8.0 and $20^{\circ}C$, respectively. The activity of the enzyme was inhibited by hydrogen peroxide, but inhibited less by cyanide and azide. The native enzyme was found to contain 0.89g-atom of iron, 0.75g-atom of zinc, and 0.46g-atom of copper per mol of enzyme. Analysis of amino acids composition revealed that the SOD from L. edodes contained a relatively large amount of glutamic acid/glutamine, proline, cysteine, isoleucine, and leucine, but only a small amount of aspartic acid/asparagine, tyrosine, and tryptophan when compared to the other iron-containing SODs.

키워드

참고문헌

  1. Phyiol. Plant v.90 Characterization of an iron-containing superoxide dismutase from a higher plant Almansa, M. S.;L. A. del Rio;F. Sevilla
  2. Biochem. v.96 The gel filtration behavior of porteins related to their molecular weight over a wide range Andres, P.
  3. Arch. Biochem. Biophys. v.280 Tetrameric manganese superoxide dismutases from anaerobic Actinomyces Barkley, K. B.;E. M. Gregory
  4. Anal. Biochem. v.44 Superoxide dismutase: Improved assays and an applicable to acrylamide gels Bcauchamp, C.;I. Fridovich
  5. Annu. Rev. Physiol. Plant. Mol. Biol. v.43 Superoxide dismutase and stress tolerance Bowler, C.;M. Van Montagu;D. Inze
  6. Anal. Biochem. v.72 A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M.
  7. Edible Mushrooms and Their Cultivation Chang, S. T.;P. G. Miles
  8. J. Microbiol. Biotechnol. v.8 False positive SOD activity of Bifidobacterium spp. grown in MRS medium Chang, W.-S.;J.-S. So
  9. Free Rad. Res. Commun. v.12;13 Nutritional effect and expression of SODs: Induction and gene expression; diagnostics; prospective protection against oxygen toxicity del Rio, L. A.;F. Sevilla;L. M. Sandalio;J. M. Palma
  10. Arch, Biochem. Biophys. v.243 Purification and characterization of an Iron-Containing Superoxide Dismutase from a Eukaryote, Ginkgo Biloba Duke, M. V.;M. L. Salin
  11. Science v.20 The biology of oxygen radicals Fridovich, I.
  12. Annu. Rev. Biochem. v.64 Superoxide radicals and Superoxide dismutases Fridovich, I.
  13. Biochim. Biophys. Acta v.289 The purification and properties of usperoxide dismutase from Saccharomyces cerevisiae Goscin, S. A.;I. Fridovich
  14. Free Radicals in Biology and Medicine (2nd ed.) Halliwell, B.;John, M. C. Gutteridge
  15. Infec. Immun. v.65 Biochemical comparison of the Cu, Zn Superoxide distamutases of Cyptococcus neoformans var. neoformans and Cryptococcus neoformans var. gattii. Hamilton, A. J.;M. D. Holdom
  16. Arch. Biochem. Biophys. v.199 Inhibitors of Superoxide dismutase: A cautionary tale Hassan, H. M.;H. Pougherty;I. Fridovich
  17. Free Rad. Res. v.22 Purification, N-terminal amino acid sequence and partial characterization of a Cu, Zn superoxide dismutase from the pathogenic fungus Aspergillus fumigatus Holodom, M. D.;A. J. Hamilton
  18. Infec. Immun. v.64 The Cu, Zn Superoxide dismutase of Aspergillus flavus, Aspergillus niger, Aspergillus nidulans, Aspergillus terreus: Purification and biochemcial comparison with the Aspergillus fumigatus Cu, Zn Superoxide dismutase Holdom M. D.;R. J. Hay;A. J. Hamilton
  19. Anim. Sci. Technol. v.64 Isolation and characterization of superoxide dismutase from Lactococcus lactis subsp. cremoris R-48 Hosno, A.;N. Satake
  20. Biochim. Biophys. Acta v.1427 Copper- and zinc-containing superoxide dismutase and its gene from Candida albicans Hwang, C.-S.;G. Rhie;S.-T. Kim;Y.-R. Kim;W. K. Huh;Y.-U. Baek;S.-O. Kang
  21. Clin. Diagn. Lab. Immunol v.5 Extracellular and cystsolic iron superoxide dismutase from Mycobacterium bovis BCG Kang, S.-K.;Y.-J. Jung;C.-H. Kim;C.-H. Song
  22. Agric. Biol. Chem. v.55 Purification and characterization of superoxide dismutase from Aerobacter aerogenes Kim, S-W.;S-O. Lee;T.-H. Lee
  23. Arch. Biochem. Biophys. v.210 Isolation and characterization of the iron-containing superoxide dismutase of Methanobacterium bryantii Kirby, T. W.;J. R. Jr., Lancaster;I. Fridovich
  24. J. Bacteriol. v.173 Copper-zinc superoxide dismutase of Haemophilus influenza and H. parainfluenza Kroll, J. S.;P. R. Langford;B. M. Loynds
  25. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli, U. K.
  26. Biochimie v.57 Purification and study of two superoxide dismutase of the mushroom Pleurotus olearius Lavelle, F.;A. M. Michelson
  27. J. Microbiol. Biotechnol. v.7 Immunostimulating activity of polysaccharides from mycelia of Phellinus linteus grown under different culture conditions Lee, J.-H.;S.-M. Kin;H.-M. Kim;N.-D. Hong;I.-D. Yoo
  28. Agric. Biol. Chem. v.52 Purification and properties of superoxide dismutase from Bacillus circulans Lee, T.-H.;S.-O. Lee
  29. J. Biol. Chem. v.260 Primary structure of copper zinc superoxide dismutase from Nerospora crassa Lerch, K.;E. Schenk
  30. J. Biol. Chem. v.244 Superoxide dismutase: An enzymic function for erythrocuprein McCord, J. M.;I. Fridovich
  31. FEBS Lett. v.103 Intracellular distribution of manganese and ferric superoxide dismutase in blue-green algae Okada, S.;S. Kanematsu;K. Asada
  32. Biochem. Mol. Biol. Int. v.42 Purification and characterization of manganese superoxide dismutase from Ganoderma microsporum Pan, S. M.;J. S. Ye;R. S. Hseu
  33. Photochem. Photobiol. v.37 Superoxide radicals. superoxide dismutases and oxygen toxicity in plants Rabinowitch, H. D.;I. Fridovich
  34. Biochim. Biophys. Acta v.1426 Maganese-containing superoxide dismutase and its gene from Candida albicans Rhie, G.;C.-S. Hwang;M. J. Brady;S.-T. Kim;Y.-R. Kim;W.-K. Huh;Y.-U. Baek;B.-H. Lee;J.-S. Lee;S.-O. Kang
  35. Arch. Biochem. Biophys. v.260 Purification of a manganese-containing superoxide dismutase from halobacterium halobium Salin, M. L.;D. Oesterhelt
  36. Biochim. Biophys. Acta v.670 Superoxide dismutase from the Archaebacterium Thermoplasma acidophilum Searcy, K. B.;D. C. Searcy
  37. J. Microbiol. Biotechnol. v.8 Activities of oxidative enzymes related with oxygen tolerace in Bifidobacterium sp. Shin, S.-Y.;J.-H. Park
  38. J. Biol. Chem. v.248 An iron-containing superoxide dismutase from Escherichia Yost, F. J.;I. Fridovich
  39. J. Biol. Chem. v.248 An iron-containing superoxide dismutase from Escherichia coli. Yost,F.J.;I.Fridovich