Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of a Pseudomonas sp. Strain Exhibiting Unusual Behavior of Poly(3-hydroxyalkanoates) Biosynthesis and Characterization of Synthesized Polyesters

  • Published : 1999.12.01
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Abstract

A Pseudomonas sp. strain that is capable of utilizing dicarboxylic acids as a sole carbon source was isolated from activated sludge by using the enrichment culture technique. This organism accumulated polyhydroxyalkanoates (PHAs) with an unusual pattern of monomer units that depends on the carbon sources used. Polyhydroxybutyrate (PHB) homopolyester was synthesized from glucose or small $C_{-even}$ alkanoic acids, such as butyric acid and hexanoic acid. Accumulation of PHB homopolyester was also observed in the cells grown on $C_{-odd}$ dicarboxylic acids, such as heptanedioic acid and nonanedioic acid as the sole carbon sources. In contrast, a copolyester consisting of 6 mol% 3-hydroxybutyrate (3HB) and 94 mol% 3-hydroxyvalerate (3HV) was produced with a PHA content of as much as 36% of the cellular dry matter. This strain produced PHAs consisting both of the short-chain-length (SCL) and the medium-chain-length (MCL) 3-hydroxyacid units when heptanoic acid to undecanoic acid were fed as the sole carbon sources. Most interestingly, polyester consisting of significant amount of relevant fractions, 3HB, 3HV, and 3-hydroxyheptanoate (3HHp), was accumulated from heptanoic acid. According to solvent fractionation experiments, the polymer produced from heptanoic acid was a blend of poly(3HHp) and of a copolyester of 3HB, 3HV, and 3HHp units. The hexane soluble fractions contained only 3HHp units while the hexane-insoluble fractions contained 3HB and 3HV units with a small amount of 3HHp unit. The copolyester was an elastomer with unusual mechanical properties. The maximum elongation ratio of the copolyester was 460% with an ultimate strength of 10 MPa, which was very different from those of poly(3HB-co-3HV) copolyesters having similar compositions produced from other microorganisms.

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References

  1. Int. J. Biol. Macromol. v.16 Biosynthesis from gluconate a random copolyester consisting of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by Psuedomonas sp. 61-3 Abe, H.;Y. Doi;T. Fukushima;H. Eya
  2. Microbiol. Rev. v.54 Occurrence, metabolism, metabolic role and industrial uses of bacterial polyhydroxyalkanoates Anderson, A. J.;E. A. Dawes
  3. FEMS Microbiol. Rev. v.103 Physical properties of poly(hydroxybutyrate) and copolymers of hydroxybutyrate and hydroxyvalerate Barham, P. J.;P. Barker;S. J. Organ
  4. Eur. J. Appl. Microbiol. Biotechnol. v.6 A rapid method for the determination of poly-β-hydroxybutyric acid in microbial biomass Braunegg, G.;B. Sconnleitner;R. M. Lafferty
  5. Int. J. Biol. Macromol. v.17 Bioshynthesis and characterization of hydroxybutyrate-hydroxycaprorate copolymers Caballero, K. P.;S. F. Karel;R. A. Register
  6. Appl. Environ. Microbiol. v.60 Polyester biosynthesis characteristics of Pseudomonas citronellosis grown on various carbon sources including 3-methyl-branched substrates Choi, M. H.;S. C. Yoon
  7. Microbial Polyesters Solid state properties of copolyesters Doi, Y.
  8. Can. J. Microbiol. v.41 no.SUP. 1 Formation of novel poly(hydroxyalkanoates) from long-chain fattyacids Eggink, G.;P. de Waard;G. N. M. Huijberts
  9. PHYLIP (Phylogeny Inference Package) vesion 3.5.1. Felsenstein, J.
  10. Biotechnol. Lett. v.19 Biosyntheis of poly(3-hydroxybutyrate-co-hydroxyvalerate-co-3-hydroxyheptanoate) terpolymers by recombinant Alcaligenes eutrophus Fukui, T.;T. Kichise;Y. Yoshida;Y. Doi
  11. Appl. Environ. Microbiol. v.60 Cultural and phylogenetic analysis of mixed microbial populations found in natural and commercial bioleaching environments Goebel, B. M.;E. Stackebrandt
  12. Appl. Environ. Microbiol. v.56 Accumulation of a polyhydroxyalkanoate containing primarily 3-hydroxydecanoate from simple carbohydrate substrates by Pseudomonas sp. strain NCLMB 40135 Haywood, G. W.;A. J. Anderson;D. F. Ewing;E. A. Dawes
  13. Appl. Environ. Microbiol. v.58 Pseuodmonas putida KT2442 cultivated on glucose accumulates poly(3-hydroxyalkanoates) consisting of saturated and unsaturated monomers Huibertsm G. N.;G. Eggink;P. de Waardl;G. W. Huisman;B. Witholt
  14. Mammarian Protein Metabolism v.3 Evoloution of protein molecules Jukes, T. H.;C. R. Cantor;H. N. Munro (ed.)
  15. Appl . Microbiol. Biotechnol. v.45 Production of a novel copolyester of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids by Pseudomonas sp. 61-3 from sugar Kato, M.;H. J. Bao;C.-K. Kang;T. Fukui;Y. Doi
  16. J. Microbiol. Biotechnol. v.7 Effect of C/N ratio on the production of poly(3-hydroxyalkanoates) by the methylotroph paracoccus denitrificans Kim, B. K.;S. C. Yoon;J. D. Nam;R. W. Lenz
  17. Macromolecules v.31 Bacterial poly(3-hydroxyalkanoates) bearing carbon-carbon triple bonds Kim, D. Y.;Y. B. Kim;Y. H. Rhee
  18. Macromolecules v.29 Poly-3-hydroxyalkanoates produced from Pseudomonas oleovorans grown with ω-phenoxyalkanoates Kim, Y. B.;Y. H. Rhee;S. H. Han;G. S. Heo;J. S. Kim
  19. Syst. Zool. v.18 Quantitative phyletics and the evolution of anurans Kluge, A. G.;F. S. Farris
  20. Appl. Environ. Microbiol. v.54 Formation of polyesters by Pseudomonas oleovorans: Effect of substrates on formation and comoposition of poly-(R)-3-hydroxyalkanoates and poly-(R)-3-hydroxyalkenoates Lageveen, R. G.;G. W. Huisman;H. Preusting;P. Ketelaar;G. Eggink;B. Witholt
  21. Nucleic Acid Techniques in Bacterial Systmatics rRNA sequencing Lane, D. J.
  22. Appl. Microbiol. Biotechnol. v.42 Biosyntheis of copolyesters consisting of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids from 1,3-butanediol or from 3-3-hydroxybutyric acid by Pseudomonas sp. A33 Lee, E. Y.;D. Jendrossek;A. Schirmer;C. Y. Choi;A. Stinbuchel
  23. FEMS Microbiol. Rev. v.103 Production of unusual bacterial polyesters by Pseudomonas oleovorans through cometabolism Lenz, R. W.;Y. B. Kim;R. C. Fuller
  24. Arch. Microbiol. v.155 Formation of poly(3-hydroxyalkanoates) by phoototrophic and chemolithotrophic bacteria Liebergessel, M.;E. Hustede;A. Timm;A. Steinbuchel;R. C. Fuller;R. W. Lenz;H. G. Schlegel
  25. Biochemical Tests for identification of Medical Bacteria (2nd ed.) Macfaddin, J. F.
  26. Macromolecules v.31 Synthesis and characterization of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) polymer mixtures produced in high-density fed-batch cultures of Ralstonia eutropha (Alcaligenes eutrophus) Madden, L. A.;A. J. Anderson;J. Astar
  27. Microbiol. Mol. Biol. Rev. v.63 Metabolic engineering of poly(3-hydroxyalkanoates): From DNA to plastic Madison, L. L.;G. W. Huisman
  28. Nucl. Acids Res. v.25 The RDT (Ribosomal Database Project) Maidak, B. L.;G. L. Olsen;N. Larsenl;R. Overbeek;M. J. McCaughey;C. Woese
  29. Bio/Technology v.13 Production of polyhydroxyalkanoates, a family of biodegradable plastics and elastomers in bacteria and plants Poirier, Y.;C. Nawrath;C. Somerville
  30. Mol. Biol. Evol. v.4 The neighbor-joining method: a new method for reconstructing phylogenetic trees Saitou, N.;M. Nei
  31. TIBTECH v.16 Bacterial and other biological systems for polyester production Steinbuchel, A.;B. Fuchtenbusch
  32. Appl. Microbiol. Biotechnol. v.37 A Pseudomonas strain accumulating polyesters of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids Steinbuchel, A.;S. Wiese
  33. Nucl. Acids Res. v.76 CLUSTAL W: Improving the sensitivity of progressive multiple sequence weighing, position-specific gap penalties and weight matrix choice Thomson, J. D.;D. G. Higgins;T. J. Gibson
  34. Appl. Environ. Microbiol. v.56 Formation of polyesers consisting of medium-chain-length 3-hydroxyalkanoic acids from gluconate by Pseudomonas aeruginosa and other fluorescent pseudomonas Timm, A.;A. Steinbuchel
  35. J. Ferment. Bioeng. v.80 Effects of amino acid additions on molar fraction of 3-hydroxyvalerate in copolyester of 3-hydroxybutyrate and 3-hydroxyvalerate synthesized by Alcaligenes sp. SH-69 Yoon, J. S.;J. Y. Kim;Y. H. Rhee
  36. Macromolecules v.28 Complex composition distribution of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Yoshie, N.;H. Menju;H. Sato;Y. Inoue
  37. Macromolecules v.28 Complex composition distribution of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Yoshie,N.;H.Menju;H.Sato;Y.Inoue