Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of a Novel Exopolysaccharide-Producing Paenibacillus sp. WN9 KCTC 8951P

  • Seo, Weon-Taek (Department of Food Processing, Chinju National University) ;
  • Kahng, Goon-Gjung (Department of Food Processing, Chinju National University) ;
  • Nam, Sang-Hae (Department of Food Processing, Chinju National University) ;
  • Choi, Sang-Do (Department of Food Processing, Chinju National University) ;
  • Suh, Hyun-Hyo (Department of Environmental Engineering, Chinju National University) ;
  • Kim, Seon-Won (Bioprocess Technology Research Division, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Yong-Ha (Korean Collection for Type Culture, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 1999.12.01
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Abstract

A bacterial strain WN9, which produced a new type of extracellular polysaccharide, was isolated from soil samples. By morphological, physiological, biochemical, and phylogenetic studies, strain WN9 was identified as a Paenibacillus sp. and it was named as Paenibacillus sp. WN9, which produced a high molecular extracellular polysaccharide from glucose. The molecular weight of the exopolysaccharide (EPS-WN9) was estimated to be about 31.5 mega-Da. The FT-IR spectrum of EPS-WN9 revealed typical characteristics of polysaccharides. EPS- WN9 consisted of D-glucose and D-mannose with a molar ratio of 1:1.4 being identified as a neutral sugar component. The acidic component of EPS- WN9 was tyrosine. Rheological analysis of EPS- WN9 revealed that the pseudoplastic property and its apparent viscosity remained stable at various temperatures and pHs.

Keywords

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