Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Purification and Characterization of Metalloproteases from Pleurotus sajor-caju

  • Published : 1999.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

Fibrinolytic protease activity was detected in the fruit body of Pleurotus sajor-caju using a fibrin plate method. Two fibrinolytic activities (FPI and Ⅱ) were found at the regions of 14.5 and 86.0 kDa by using gel-filtration column chromatography. FPⅡ was identified as an alkaline protease, whereas FPⅠ was a neutral protease. Both were inhibited by phenanthrolin and EDTA, suggesting that they are metalloprotease. Inactivated enzyme activities were restored by adding Co/sup 2+/ or Zn/sup 2+/. Iodoacetate inhibited FPⅠ, but not FPⅡ. Both enzymes cleaved B/sub β/ and γ chains of the human fibrinogen. FPⅡ showed a preference to hydrophobic and bulky residues of nitroanilidine compounds as substrates, whereas FPⅠ preferred positively charged residues.

Keywords

References

  1. Arch. Biochem. Biophys. v.334 A developmental control of cathepsin B expression in bovine fetal muscle Bechet, D. M.;C. Deval;J. Ribellin;M. J. Ferrara;A. Obled
  2. Mycologia v.90 Purification and partial characterization of a fibrinolytic protease in Pleurotus ostreatus Choi, H.-S.;H.-H. Shin
  3. Biochem. Cell Biol. v.65 Cell growth and substrate effects on characteristics of a lysosomal enzyme in Duchenne muscular dystropy fibrolasts Doughty, M. J.;E. I. Gruenstein
  4. Vet. Parasitol. v.62 Characterization of excretory-secretory products from larval stages of Haemonchus contortus cultured in vitro Gamble, H. R.;L. S. Mansfield
  5. Arch. Biochem. Biophys. v.311 Purification and characterization of a natural zinc endopeptidase secreted by Flavobacterium meningosepticum Grimwood, B. G.;T. H. Plummer, Jr.;A. L. Tarentino
  6. Plant Physiol. v.99 Purification and characterization of aleurain Holwerda, B. C.;J. C. Rogers
  7. J. Biochem. Mol. Biol. v.28 An anticoagulant/fibrinolytic protease from Lumbricus rubellus Jeon, O.-H.;W.-J. Moon;D. S. Kim
  8. J. Microbiol. Biotechnol. v.8 Degradation of polycyclic aromatic hydrocarbons by selected white-rot fungi and the influence of lignin peroxidase Kim, M. S.;E. J. Huh;H. K. Kim;K. W. Moon
  9. J. Microbiol. Biotechnol. v.8 Characterization of alkaline serine proteases secreted from the Coryneform bacterium TU-19 Kang, S. C.;S. Park;M. C. Choi
  10. Biosci. Biotech. Biochem. v.61 Purification and properties of a cystein protease occurring in dormant wheat seeds Kuroda, M.;T. Kiyosaki;S. Arai;K. Abe
  11. Nature v.227 Cleavage of structural proteins dudring the assembly of the head of bacteriophage T4 Laemmli, U. K.
  12. Agric. Biol. Chem. v.45 Purification and some properties of carboxyl proteinase in mycelium of Lentinus edodes Terashita, T.;K. Oda;M. Kono;S. Murao
  13. Agric. Biol. Chem. v.49 Purification and some properties of metal proteinases from Lentinus edodes Terashita, T.;K. Oda;M. Kono;S. Murao
  14. Eur. J. Biochem. v.244 Overexpression of cruzipain, the major cystein proteinase of Tripanosoma cruzi, is associated with enhanced metacyclogenesis Tomas, A. M.;M. A. Miles;J. M. Kelly