Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Discovery of D-Stereospecific Dipeptidase from Thermophilic Bacillus sp. BCS-l and Its Application for Synthesis of D-Amino Acid-Containing Peptide

  • Baek, Dae-Heoun (Microbial Conversion Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwon, Seok-Joon (Microbial Conversion Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Park, Jin-Seo (Microbial Conversion Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Seung-Goo (Microbial Conversion Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Mheen, Tae-Ick (Microbial Conversion Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Sung, Moon-Hee (Microbial Conversion Research Unit, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • Published : 1999.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

A thermophilic bacterium producing D-stereospecific dipeptidase was isolated from Korean soil samples. The enzyme hydrolyzed the peptide bond between D-alanyl-D-alanine (D-Ala-D-Ala). The isolated bacterial strain was rod shaped, gram-positive, motile, and formed an endospore. Morphological and physiological characteristics suggested this microorganism a thermophilic Bacillus species, and was named as Bacillus sp. BCS-l. The production of D-stereospecific dipeptidase was growth-associated and optimal at $55^{\circ}C$. The enzyme was applied for the synthesis of D-amino acid-containing peptide, N-benzyloxycarbonyl-L-aspartyl-D-alanine benzyl ester (Z-L-Asp-D-AlaOBzl), as a model reaction. A thermodynamically controlled synthesis of Z-L-Asp-D-AlaOBzl was achieved in an organic solvent.

Keywords

References

  1. Clin. Chem. v.20 Enzymatic determination of total serum cholesterol Allain, C. C.;L. S. Poon;C .S. G. Chan;W. Richmond;P. Fu
  2. J. Biol. Chem. v.271 An alkaline D-stereospecific endopeptidase with β-lactamase activity from Bacillus cereus Asano, Y.;H. Ito;T. Dairi;Y. Kato
  3. Angew. Chem. Int. Ed. Engl. v.28 Deiscovery of D-stereospecific aminopeptidase and its use as a catalyst in organic synthesis Asano, Y.;A. Nakazawa;Y. Kato;K. Kondo
  4. J. Biol. Chem. v.264 Properties of a novel D-strerospecific aminopeptidase from Ochrobacterum anthropi Asano, Y.;A. Nakazawa;Y. Kato;K. Kondo
  5. Proc. Natl. Acad. Sci. USA v.85 Highly potent antagonists of luteinizing hormone-releasing hormone free of edematogenic effects Bajusz, S.;M. Kovacs;M. Gazdag;L. Bokser;T. Karashima;V. J. Csernus;T. Janaky
  6. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding Bradford, M. M.
  7. Manual for the Identification of Medical Bacteria(2nd ed.) Characters of gram-negative bacteria Cowan, S. T.
  8. Durg Metab. Dispos. v.21 Posttranslational amino acid epimerization: Enzyme catalyzed isomerization of amino acid residues in peptide chains Heck, S. D.;W. S. Kelbaugh;N. A. Saccomano;P. F. Thadeio;R. A. Volkmann
  9. J. Med. Chem. v.33 High potency dipeptide sweeteners. 1. L-Asp-D-Phenylglycine esters Janusz, J. M.;J. M. Gardlik;P. A. Young;R. V. Burkes;S. J. Stoll;A. F. Estelle;C. M. Riley
  10. Science v.194 D-Ala²-Met-enkephalineamide: A potent, long-lasting synthetic pentapeptide analgesic Pert, C. B.;A. Pert;J. K. Chang;B. T. W. Fong
  11. Life Sci. v.19 D-Amino acids in higher plants Rhobinson, T.
  12. Bacteriol. Rev. v.36 Peptidoglycan types of bacterial cell walls and their taxonomical implications Schleifer, K. H.;O. Kandler
  13. FASEB J. v.11 Different roles of D-amino acids in immune phenomena Sela, M.;E. Zisman
  14. Thermophilic Bacteria Heterotrophic thermophilic Bacilli Sharp, R. J.;P. W. Riley;D. White;J. K. Kristjacsson(ed.)
  15. Bergey's Manual of Systematic Bacteriology v.2 Endospore-forming gram positive rods and cocci Sneath, P. H. A.;Sneath, P. H. A.(eds.);N. S. Mair(eds.);Elisabeth Sharpe(eds.);J. G. Holt(eds.)
  16. Agric. Biol. Chem. v.50 Purification and properties of a peptidase from Nocardia orientalis specific to D-amino acid peptides Sugie, M.;H. Suzuki;N. Tomizuka
  17. The prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria v.1 The geunes Alcaligenes Tilyon, R. C.;Starr, M. P.(eds.);H. Stopl(eds.);H. C. Truper(eds.)A. Balows(eds.);H. G. Schlegel(eds.)
  18. Biochemistry v.34 Overexpression, purification, and characterization of Van X, a D-,D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147 Wu, Z.;G. D. Wright;C. T. Walsh
  19. Biochemistry v.34 Overexpression, purification, and characterization of Van X, a D-,D-dipeptidase which is essential for vancomycin resistance in Entercoccus faecium BM4147 Wu,Z.;G.D.Wright;C.T.Walsh