Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Hyper-CMCase-Producing Mutants of Bacillus sp. 79-23 Induced by Gamma- Radiation

  • Published : 1999.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Bacillus sp. 79-23 spores were irradiated with $^{60}Co$ gamma-rays at doses ranging from 0.5 to 5 kGy. Following gamma-irradiation, seven mutant strains were isolated by scoring the halo sizes formed around the colonies grown on LB agar plates containing 4% carboxymethylcellulose (CMC) and trypan blue. The mutant strains showed a 1.5 to 2-fold increase in carboxymethylcellulase (CMCase) activity over the parent strain. Wheat bran acted as an effective inducer for CMCase production in the parent and mutant strains. Mutant strains 68 and 70 were identified as exhibiting higher CMCase activities than those of other mutants in LB media both with and without 3% wheat bran. In addition, these strains seem to produce substantially lower amounts of capsular materials, whereas the parent strain produced large amounts of them in both liquid and solid LB media. In flask cultures, the CMCase production by mutants 68 and 70 reached maximum levels of 17.5 unit/ml and 15.7 unit/ml, respectively, in an LB medium containing 3% wheat bran.

Keywords

References

  1. Appl. Microbiol. Biotechnol v.46 Saccharomyces cerevisiae Mutants selected for increased production of Trichoderma reesei cellulases Aho, S.;A. Arffman;M. Korhola
  2. Annu. Rev. Microbiol v.44 Molecular biology of cellulose degradation Beguin, P
  3. FEMS Microbiol. Rev v.13 The biological degradation of cellulose Beguin, P.;J.-P. Aubert
  4. J. Gen. Microbiol v.139 Bacterial cellulases and xylanases Gilbert, H. J.;G. P. Hazlewood
  5. FEBS Lett v.281 DNA damage by dxygen-derived species.Its muchanism and measurement in mammlian cells Halliwell, B.;O. I. Aruoma
  6. J. Gen. Appl. Microbiol v.44 Integration and amplification of the Bacillus so. 79-23 cellulase gene in the Bacillus subtilis 168 chromosome Jung, K. H.;D.-H. Lee;K.-H. Yoon;S.-H. Park
  7. Biotechnol. Lett v.18 Cloning and expression of a Bacillus so.79-23 cellulase gene Jung, K. H.;Y. C. Chun;J.-C. Lee;J. H. kim;K.-H. Yoon
  8. Appl. Environ. Microbiol v.62 Mechanism by which gamma irradiation increases the sensitivity of Slmonella typhimurium ATCC 14028 to heat Kim, A. Y.;D. W. thayer
  9. J. Microbiol. Biotechnol v.8 Strain improvement of yeast for ethanol production using a combines treatment of electric field and chemical mutagen N-methyl-N-nitro-N-nitrosoguanidine Kim, K.;J. Y. Lee
  10. Lett. Appl. microbiol v.19 A hypercellulolytic mutant of Fusarium oxysporum Kuhad, R. C.;M. Kumar;A. Singh
  11. J. Gen. Microbiol v.121 Generalized transduction in Bacillus thuringiensis var berliner 1715 using bacteriophage CP-54 Ber Lecadet, M.-M.;M.-O. Blondel;J. Ribier
  12. Rad. Res v.74 A model for radiation damage in cells by direct effect and by indirect effect: A radiation chemistry approach Michaels, H. B.;J. W. Hunt
  13. Anal. Biochem v.2 Measurement of carboxymethylcellulase activity Miller, M. L.;R. Blum;W. E. Glennon;A. L. Burton
  14. J. Microbiol. Biotechnol v.8 Development of cellobiose-utilizing recombinant yeasr for ethanol production from cellulose hydrolyzate Pack, S. P.;K. M. Cho;H.-S. Kang;Y. J. Yoo
  15. Crit. Rev. Biotechnol v.17 Xylanolytic enzymes from fungi and bacteria Sunna, A.;G. Antranikian
  16. Adv. Microb. Physiol v.37 Cellulose hydrolysis by bacteria and fungi Tomme, P.;R. A. J. Warren;N. R. Gilkes
  17. The Chemical Basis of Radiation Biology von Sonntag, C.
  18. Mutat. Res v.408 Influence of the UV-activated SPS response in the γ-radiation-induced mutation spectrum in the lacl gene Wijker, C. A.;M. V. M. Lafleur
  19. Mutat. Res v.403 Mutation spectrum in the lacI gene, induced by γ-radiation in aqueous solution under toxic conditions Wijker, C. A.;N. M. Wientjes;M. V. M. Lafleur
  20. Kor. J. Appl. microbiol. Biotechnol v.25 Isolation and enzyme production of a cellulase=producing Bacillus sp. 79-23 Yoon, K.-H.;K. H. Jung;S.-H. Park
  21. J. Microbiol. Biotechnol v.8 Production of cellulase by Trichoderma reesei Rut C30 in Wheat brancontaining media Yu, X.-B.;H. S. Yun;Y.-M. Koo