Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Circular Permutation of the DNA Genome of Temperate Bacteriophage $\PhiFC1$ from Enterococcus faecalis KBL 703

  • Kim, Young-Woo (Graduate School of Biotechnology, Korea University) ;
  • Jang, Se-Hwan (Graduate School of Biotechnology, Korea University) ;
  • Hong, Bum-Shik (Graduate School of Biotechnology, Korea University) ;
  • Lim, Wang-Jin (Graduate School of Biotechnology, Korea University) ;
  • Kim, Chan-Wha (Graduate School of Biotechnology, Korea University) ;
  • Sung, Ha-Chin (Graduate School of Biotechnology, Korea University) ;
  • Chang, Hyo-Ihl (Graduate School of Biotechnology, Korea University)
  • Published : 1999.08.01
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Abstract

The physical map of bacteriophage $\PhiFC1$ DNA was constructed with the restriction endonucleases SalI, BamHI, EcoRI, XbaI, and AvaI. The 40.5-kb DNA restriction map is shown to be circularly permuted representing the headful packaging mechanism of the phage. The DNA restriction fragments containing the packaging initiation site(pac) was localized on the restriction map and the nucleotide sequences of the region were analyzed. Four open reading frames (ORFs), following one another with the same orientation, were found at the region. The 2nd ORF (ORF-ts) has significant amino acid sequence homologies to the previously known terminase small subunits of other bacteriophages. The putative terminase small subunit gene has a presumptive NTP-hydrolysis motif and a helix-turn-helix motif. The cleavage site for the first round of packaging was found to be located at the coding sequence of the putative terminase small subunit gene. The fourth ORF, even if partially sequenced, has a good amino acid sequence homology to the portal vertex proteins of other bacteriophages representing the evolutionarily conserved arrangements of genes near the pac site of this bacteriophage, $\PhiFC1$.

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