Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Symmetry Region at Beginning of Transcript Inhibits Expression of Escherichia coli aeg-46.5 Operon

  • Lee, Seung-Hwa (Department of Chemistry, Graduate School of Biotechnology, Korea University) ;
  • Lee, Sang-Ho (Department of Chemistry, Graduate School of Biotechnology, Korea University) ;
  • Sung, Ha-Chin (Department of Chemistry, Graduate School of Biotechnology, Korea University) ;
  • Kim, Joon (Department of Chemistry, Graduate School of Biotechnology, Korea University) ;
  • Choe, Mu-Hyeon (Department of Chemistry, Graduate School of Biotechnology, Korea University)
  • Published : 1999.08.01
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Abstract

The aeg-46.5 operon of Escherichia coli is induced by nitrate and anaerobic conditions. Positive regulators Fnr and NarP, and a negative regulator NarL control the expression of the aeg-46.5. It has two symmetry regions [6], one of which is located between +37 and +56 bp from the 5'end of the anaerobic transcription initiation site. In this study, mutagenized symmetry regions were transferred from plasmid to chromosome by homologous recombination to evaluate the mutation as a single copy in the fnr, narL, narP, and narL-narP double mutant background. The expressions of the aeg-46.5 operon with these mutations indicated that the control was not through the possible stem-loop structure. Whether there is a protein that mediates this control remains to be seen. The results from the narL-narP double mutant indicated that the anaerobic Fill induction was independent of NarL repression.

Keywords

References

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