Responses of Pseudomonas sp. DJ-12 to Pollutant Stresses of Benzoate and 4-Chlorobenzoate

  • Ko, Yeon-Ja (Department of Microbiology and Research Institute of Genetic Engineering, Chungbuk National University) ;
  • Park, Sang-Ho (Department of Microbiology and Research Institute of Genetic Engineering, Chungbuk National University) ;
  • Park, Yong-Keun (Graduate School of Biotechnology, Korea University) ;
  • Kim, Chi-Kyung (Department of Microbiology and Research Institute of Genetic Engineering, Chungbuk National University)
  • 발행 : 1999.08.01

초록

Aromatic hydrocarbons can be utilized as carbon and energy sources by some microorganisms at lower concentrations. However, they can also act as stresses to these organisms at higher concentrations. Pseudomonas sp. DJ-12 is capable of degrading 0.5 mM concentration of benzoate and 4-chlorobenzoate (4CBA). In this study, the exposure of Pseudomonas sp. DJ-12 to the pollutant stresses of benzoate and 4CBA at various concentrations was comparatively studied for its cellular responses, including survival tolerance, degradability of the aromatics, and morphological changes. Pseudomonas sp. DJ-12 utilized 0.5 to 1.0mM benzoate and 4CBA as carbon and energy sources for growth. However, the organism could not degrade the aromatics at concentrations of 3 mM or higher, resulting in reduced cell viability due to the destruction of the cell envelopes. Pseudomonas sp. DJ-12 cells produced stress-shock proteins such as DnaK and GroEL when treated with benzoate and 4CBA at concentrations of 0.5mM, or higher as sublethal dosage; Yet, there were differing responses between the cells treated with either benzoate or 4CBA. 4CBA affected the degradability of the cells more critically than benzoate. The DnaK and GroEL stress-shock proteins were produced either by 1mM benzoate with 5 min treatment or by higher concentrations after 10min. The proteins were also induced by 0.5mM 4CBA, however, it needed at least 20min treatment or longer. These results indicate that the chlorination of benzoate increased the recalcitrance of the pollutant aromatics and changed the conditions to lower concentrations and longer treatment times for the production of stress-shock proteins. of stress-shock proteins produced by the aromatics at sublethal concentrations functioned interactively between the aromatics for survival tolerance to lethal concentrations.

키워드

참고문헌

  1. Appl. Environ. Microbiol v.60 Formation of chlorocatechol meta cleavage products by a pseudomonad during metabolism of monochlorobiphenyls Arensdorf, J. J.;D. D. Focht
  2. Appl. Environ. Microbiol v.62 Oxidative stress detection with Escherichia coli harboring a katG'ːːlux fusion Belkin, S.;D. R. Smulski;A. C. Vollmer;T. K. Van Dyk;R. A. LaRossa
  3. Appl. Environ. Microbiol v.63 Evidence that formation of protoanemonin from metabolites of 4-chlorobiphenyl degradation negatively affects the survival of 4-chlorobiphenyl-cometabolizing microorganisms Blasco, R.;M. Mallavarapu;R. M. Wittich
  4. Appl. Environ. Microbiol v.58 Unique and overlapping pollutant stress proteins of Escherichia coli Blom, S.;W. Harder;A. Matin
  5. Protein Methods (3rd ed.) Bollag, D. M.;M. D. Rozycki;S. J. Edelstcin
  6. J. Microbiol v.35 Dcchlorination of 4-chlorobenzoate by Pseudomonas sp. DJ-12 Chae, J. C.;C. K. Kim
  7. Microbiol. Rev. v.55 Biodegradation of halogenated organic compounds Chaudhry, G. R.;S. Chapalamadugu
  8. Appl. Environ. Microbiol v.58 Physiological properties of a Pseudomonas strain which grows with P-xylene in a two-phase (Organic-aqueous) medium Crude, D. L.;J. H. Wolfram;R. D. Rogers;D. T. Gibson
  9. FEMS Microbiol. Lett v.145 Impaired oxidative stress resistance of Bacillus subtilis sigB mutants and the role of katA and katE Engelmann, S.;M. Hecker
  10. FEMS Microbiol. Lett v.111 Transient repression of the synthesis of OmpF and aspartate transcarbamoylase in Escherichia coli K12 as a response to pollutant stress Faber, F.;T. Egli;W. Harder
  11. J. Gen. Microbiol v.139 Saccharomyces cerevisiae has an inducible response to menadione which differs from that to hydrogen peroxide Flattery-O'Brien, J.;L. P. Collinson;I. W. Dawes
  12. J. Bacteriol v.175 The acid tolerance response of Slmonella typhimurium involves transient synthesis of key acid shock proteins Foster, F. W.
  13. Appl. Environ. Microbiol v.35 Effect of chlorine substitution on the biodegradability of polychlorinated biphenyls Furukawa, K.;K. Tonomura;A. Kamibayashi
  14. J. Bacteriol v.175 Adaptation of Escherichia coli to the uncoupler of oxidative phosporylation 2,4-dinitrophenol Gage, D. J.;F. C. Neidhardt
  15. Cell v.69 Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor $O^32$ in Escherichia coli Gamer, J.;H. Bujard;B. Bukau
  16. J. Bacteriol v.176 Induction of heat shock proteins by abnormal proteins results from stabilization and not increased synthesis of $O^32$ in Escherichia coli Kanemori, M.;H. Mori;T. Yura
  17. Appl. Environ. Microbiol v.63 Induction of heat shock proteins Dnak, GroEL, and GroES by salt stress in Lactococcus lactis Kilstrup, M.;S. Jacobsen;K. Hammer;F. K. Vogensen
  18. Kor. J. Microbiol v.25 Isolation and characterization of bacteria degrading chlorinated aromatic hydrocarbons Kim, J. W.;C. K. Kim;J. H. Yeoum;J. G. Lee
  19. Kor. J. Microbiol v.32 Cloning and expression of pcbCD genes in Escherichia coli from Pseudomonas sp. DJ-12 Kim, C. K.;T. K. Sung;J. H. Nam;Y. C. Kim;J. K. Lee
  20. Nature v.356 Successive action of DnaK, DnaJ, and GroEL along the pathway of chaperone-mediated protein folding Langer, T.;C. Lu;H. Echols;J. Flanagan;M. K. Hayer;F. U. Harti
  21. Mol. Microbiol v.5 Physiological roles of the DnaK and GroE stress proteins: Catalysts of protein folding or macromolecular sponges? LaRossa, R. A.;T. K. Van Dyk
  22. Toxicol. Appl. Pharmacol v.142 Toluence disrupts outer hair cell morphometry and intracellular calcium homeostasis in cochlear cells of guinea pigs Liu, Y.;L. D. Fechter
  23. Appl. Environ. Microbiol v.61 Two-dimensional gel electrophoresis analysis of the response of Pseudomonas putida KT2442 to 2-chlorophenol Lupi, C. G.;T. Colangelo;C. A. Mason
  24. Microbiol. Rev. v.59 Stress-induced transcriptional activation Mager, W. H.;A. J. De Kruijff
  25. J. Bacteriol v.164 Morphological forms and viability of Campylobacter species studied by electron microscopy Ng, L. K.;R. Sherburne;D. E. Taylor;M. E. Stiles
  26. J. Microbiol v.36 Cellular responses of Pseudomonas sp. DJ-12 to the stresses of several aromatic pollutants Park, S. H.;Y. J. Ko;K. H. Oh;C. K. Kim
  27. J. Microbiol v.36 Production of stress-shock proteins in Pseudomonas sp. DJ-12 treated with 4-hydroxybenzoate Park, S. H.;K. H. Oh;K. J. Lee;C. K. Kim
  28. Molecular Cloning (2nd ed) Sambrook, J.;E. F. Fritsch;T. Maniatis
  29. J. Bacteriol v.269 Interaction of cyclic hydrocarbons with biological membranes Sikkema, J.;J. A. M. de Bont;B. Poolman
  30. Microbiol. Rev. v.59 Mechanisms of membrane toxicity of hydrocarbons Sikkema, J.;J. A. M. de Bont;B. Poolman
  31. J. Bacteriol v.168 Global control in Salmonella typhimurium: Two-dimensional eletropjoresis analysis of starvation-,anaerobiosis-,and heat shock-inducible proteins Spector, M. P.;Z. Aliabadi;T. Gonzalez;J. W. Foster
  32. Appl. Environ. Microbiol v.57 Effect of benzoic acid on glycolytic metabolism levels and intracellular pH in Saccharomyces cerevisiae Warth, A. D.