Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Molecular Cloning and Expression of Genes Related to Antifungal Activities from Enterobacter sp. B54 Antagonistic to Phytophthora capsici

  • YOON, SANG-HONG (Department of Molecular Genetics, National Institute of Agricultural Sciences & Technology, Rural Development Administration)
  • Published : 1999.06.01
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Abstract

Enterobacter sp. B54 inhibited growth of the fungus Phytophthora capsici on potato dextrose agar (PDA). Three mutants with antifungal activities (denoted M54-47, M54-113, and M54-329) which were lost or increased, through Pl::Tn5 lac mutagenesis, were used to isolate genes responsible for fungal inhibition on PDA. Two clones were selected from the partially EcoR1-digested genomic library of the wild-type strain by probing with genomic flanking sequences of each mutant. We have isolated a 20-kb EcoR1 genomic DNA fragment from this strain that contains genes involved in hyphal growth inhibition of P. capsici on PDA. Subcloning and expression analysis of the above DNA fragment identified a 8-kb region which was necessary for antifungal activities. A 8-kb HindⅢDNA fragment covers three genomic loci inserted by Tn5 lac in each mutant. This suggested that all genes which are related to antifungal activities might be clustered in simple forms of at least 5-8 kb sizes.

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References

  1. Phytopathology v.67 Effect of Bacillus spp. on increased growth of seedlings in steamed and in nontreated soil Broadbent, P.;K. F. Baker;N. Franks;P. Holland
  2. Current Microbiol. v.32 Pyrrolnitrin production by an Enterobacter agglomerans strain with a broad spectrum of antagonistic activity towards fungal and bacterial Phytopathogens Chernin, L.;A. Brandis; Z. Iamailov;I. Chet
  3. Appl. Environ. Microbiol. v.52 Multiple antibiotics produced by Pseudomonas fluorescens HV37a and their differential regulation by glucose Douglas, W. J. Jr.;N. I. Gutterson
  4. Annu. Rev. Phytopathol. v.26 Role of antibiosis in the biocontrol of plant disease Fravel, D. R.
  5. J. Bacteriol. v.170 Genetic determinants for the catabolite of antibiotic biosynthesis in Pseudomonas fluorescens HV37a Gutterson, N.;J. S. Ziegle
  6. J. Bacteriol. v.165 Genetic determinants for catabolite induction for antibiotic biosynthesis in Pseudomonas fluorescens HV37a Gutterson, N.;J. S. Ziegle;G. J. Warren;T. J. Layton
  7. Phytopathology v.73 Effects of pregermination of pea and cucumber seeds and of seed treatment with Enterobacter cloacae on roots caused by Pythium spp Hadar, Y.;G. E. Harman;A. G. Taylor;J. M. Norton
  8. Appl. Environ. Microbiol. v.63 Four genes from Pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin Hammer, P. E.;D. S. Hill;S. T. Lam;K. H. van Pee;J. M. Ligon
  9. Soil. Biol. Biochem. v.21 Production of antibiotics by Pseudomonas cephacia as an agent for biological control of soil borne plant pathogens Homma, Y.;Z. Sato;F. Hirayamaceta;K. Konno;H. Shirahama;T. Suzui
  10. Phytopathology v.77 Production of a volatile antibiotic by Enterobacter cloacae and its possible role in the biological control of pathogenic fungi by the bacterium Howell, C.;R. Stipanovic
  11. Plant Disease v.64 Biological control of crown gall through production of agrocin 84 Kerr. A.
  12. Plasmids v.12 Factors affecting the isolation of ccc DNA from Streptomyces lividans Escherichia coli Kieser, T.
  13. Proc. Natl. Acad. Sci. USA v.81 Construction of Tn5 lac, a transposon that fuses lacZ expression to exogenous promoters, and its introduction into Myxococcus xanthus Kroos, L.;D. Kaiser
  14. Phytopathology v.78 Role of fulorescent siderophore production in biological control of Pythium ultimum by a Pseudomonas fluorescens strain Loper, J. E.
  15. Can. J. Pant Pathol. v.14 Molecular approaches for understanding biological control mechanisms in bacteria: studies of the interaction of Enterobacer cloacae with Pythium ultimum Nelson, E. B.;A. P. Malony
  16. Can. J. Microbiol v.30 Role of antibiosis in antagonism of Streptomyces hygroscopicus var. geldanus to Rhizoctonia solani in soil Rothrock, C. R.;D. Gottlieb
  17. Antimicrob. Agents Chemother. v.32 Streptomyces cloning: Possible construction of novel compounds and regulation of antibiotic biosynthetic genes Tomich, P. K.
  18. Can. J. Bot. v.61 Inhibition of Phytophthora cactorum by a bacterial antagonist Utkhede, R. S.;A. P. Gaunce
  19. Appl. Environ. Micribiol. v.57 Genetic analysis of the antifungal activity of a soilborne Pseudomonas aureofaciens strain Vincent, M. N.;L. A. Harrison;J. M. Brackin;P. A. Kovacevich;P. Mukerji;D. M. Weller;E. A. Pierson
  20. Kor. J. Appl. Microbiol. Biotech. v.26 Tn5 lac mediated mutagenesis of Enterobacter sp. B54 antagonistic to Phytophthora capsici Yoon, S. H.;C. Choi