Journal of Microbiology and Biotechnology

  • 제9권3호
  • /
  • Pages.334-339
  • /
  • 1999
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

A Molecular Marker Specific to Metarhizium anisopliae var. majus

  • YOON, CHEOL-SIK (Research Institute of Engineering and Technology, Korea University) ;
  • GI HO SUNG (Department of Agricultural Biology, Kangweon National University) ;
  • JAE MO SUNG (Department of Agricultural Biology, Kangweon National University) ;
  • JAEANG OON LEE (Division of Entomology, National Institute of Agricultural Science and Technology, RDA)
  • 발행 : 1999.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

More innovative molecular markers were investigated for rapid and consistent differentiation of Metarhizium anisopliae var. majus from M. anisopliae var. anisopliae. A total of 28 isolates were obtained from various countries and hosts: 13 isolates of M. anisopliae var. anisopliae, 12 isolates of M. anisopliae var. majus, and 3 isolates of M. anisopliae collected in Korea. This study involved restriction enzyme digestions of a PCR product amplified from nuclear internally transcribed spacer (ITS) and a portion of the 28S rDNA regions. Among 11 different restriction enzymes used in this study, MboⅠ digestion particularly produced a restriction pattern that had characteristics of M. anisopliae var. majus. This restriction pattern was consistent in all isolates of M. anisopliae var. majus regardless of their geographic origins and insect hosts. Mapping experiments revealed that MboⅠ sites of M. anisopliae var. majus are identical to those of M. anisopliae var. anisopliae with an exception for the presence of an additional site in the PCR product. Results from this study provide an additional method for identification and differentiation of isolates of these two varieties of M. anisopliae for use in the field and laboratory experiments.

키워드

참고문헌

  1. Biocontrol Sci. Technol. v.2 Pathogenicity of the entomogenous, hyphomycete fungus, Metarhizium anisopliae against the chrysomelid beetles Psyliodes chrysocephala and Phaedon cochleriae Butt, T. M.;M. Barrisever;J. Drummond;T. H. Schuler;F. T. Tillermans;N. Wilding
  2. Mycol. Res. v.98 Phylogeny of Metarhizium: Analysis of ribosomal DNA sequence data Curran, J.;F. Driver;J. W. O. Ballard;R. J. Milner
  3. Parasitology v.84 Fungi as biological control agents of athropods of agricultural and medicai importance Hall, R. A.;B. Papierok
  4. Curr. Sci. v.21 Occurrence of the green muscardine fungus on Pyrilla species in Bombay Kamat, M. N.;M. K. Patel;G. W. Dhande
  5. Mycol. Res. v.98 Characterization of isolates of the entomopathogenic fungus Metarhizium anisopliae by RAPD-PCR Leal, S. C. M.;D. J. Bertioli;T. M. Butt;J. F. Peberdy
  6. Molecular Evolutionary Genetics Nei, M.
  7. Mycol. Res v.99 Restriction fragment length polymorphisms in the ribosomal RNA gene complex of isolates of the entomopathogenic fungus Metharhizium anisopliae Pipe, N, D.;D. Chandler;B. W. Bainbridge;J. B. Heale
  8. Mycol. Res. v.98 Phylogenetic relationships within the genus Metarhizium based on 28S rRNA sequences and isozyme comparison Rakotonirainy, M. S.;M. L. Cariou;Y. Brygoo;G. Riba
  9. Trans. Brit. Mycol. Soc. v.81 Metarhizium album, a fungal pathogen of a leaf-and planthoppers of the rice Rombach, M. C.;R. A. Humber;H. C. Evans
  10. Trans. Brit. Mycol. Soc. v.66 The genus Metarhizium Tulloch, M.
  11. J. Bacteriol. v.172 Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species Vilgalys, R.;M. Hester
  12. PCR Protocols: A Guide to Methods and Applications Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics White, T. J.;T. Bruns;S. Lee;J. Taylor;M. A. Innis(ed.);D. H. Gelfand(ed.);J. J. Sninsky(ed.);T. J. White(ed.)
  13. Mycologia v.83 A method for rapid small-scale preparation of fungal DNA Yoon, C.-S.;D. A. Glawe;P. D. Shaw