Journal of Microbiology and Biotechnology

  • 제9권3호
  • /
  • Pages.311-317
  • /
  • 1999
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Expression and Characterization of Human T-Cell Leukemia Virus Type-I Env and Gag Proteins

  • 발행 : 1999.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Human T-cell leukemia virus Type-I (HTLV-I) is etiologically associated with rare adult T-cell leukemia, a malignant T-cell disorder. cDNAs encoding p24 (gag), gp21(env), and pXII of HTLV-I were amplified by polymerase chain reaction (PCR) using the genomic DNA extracted from HUT102 cell line as a template. The amplified cDNAs were cloned into the Escherichia coli expression vectors and over-expression of the recombinant proteins were achieved by adding IPTG into the culture media in order to induce the promoter. The molecular weights of the recombinant p24, gp21, and pXII, determined by SDS-PAGE, were found to be approximately 28 kDa, 23 kDa, and 15 kDa, respectively. Reactivity of the recombinant proteins with human sera was tested by the immunoblot assay. The gp21 and p24 reacted against the sera obtained from HTLV-I-infected individuals but not against the sera obtained from normal persons. These results suggest that the recombinant proteins expressed in E. coli were recognized by antibodies in sera from HTLV-I infected patients. These recombinant proteins would be applicable for detecting the presence of antibodies against HTLV-I in human blood samples.

키워드

참고문헌

  1. Virology v.196 HTLV-I gene expression by defective proviruses in an infected T-cell line Bhat, N. K.;Y. Adachi;K. P. Samuel;D. Derse
  2. Lancet v.336 The specific antigens for serological descrimination of HTLV-I and HTLV-II infection Chen, Y. A.;T. H. Lee;S. Z. Wiktor;G. M. Shaw;E. L. Murphy;W. A. Blattner;M. Essex
  3. Blood v.86 Molecular mechanisms of human T-cell leukemia lymphotropic virus type I infection Franchini, G.
  4. Lancet v.8452 Antibodies to human T-lymphotrophic virus type-I in patients with tropical spastic parapatesis Gessain, A.;F. Barin;J. C. Vernant;O. Gout;L. Maurs;A. Calender
  5. Proc. Natl. Acad. Sci. USA v.78 Adult T-cell leukemia: Antigen in an ATL cell line and detection of antibodies to the antigen in human sera Hinuma, Y.;K. Nagata;M. Hanaoka;M. Nakai;T. Matsumoto;K. Kinoshita;S. Shirakawa;I. Miyoshi
  6. Proc. Natl. Acad. Sci. USA v.88 Identification of type specific linear epitopes in the glycoproteins gp46 and gp21 of human T-cell leukemia viruses type I and type II using synthesis peptides Horal, P.;W. W. Hall;B. Svennerholm;J. Lycke;S. Jeansson;L. Rymo;M. H. Kaplan;A. Vahlne
  7. Nature v.294 Antibodies in human sera reactive against an internal structural protein of human T-cell lymphoma virus Kalyanaraman, V.S.;M.G. Sarngadharan;P.A. Bunn;J. D. Minna;R. C. Gallo
  8. J. Clin. Microbiol. v.32 Evaluation of a p21e-spiked western blot (immunoblot) in confirming human T-cell lymphotropic virus-type I or II infection in volunteer blood donors Kleinman, S. H.;J. E. Kaplan;R. F. Khabbaz;M. A. Calabro;R. Thomson;M. Busch
  9. Transfusion v.33 Transfusion transmission of human T- lymphotropic virus type I and II serologic and polymerase chain reaction results in recipients identified through look-back investigation Kleinman, S.;P. Swanson;J. P. Allain;H. Lee
  10. Int. J. Cancer v.45 Detection of antibodies to human T-lymphotropic virus type I by using synthetic peptides Kuroda,N.;Y. Washitani;H. Shiraki:H. Kiyokawa;M. Ohno;H. Sato;Y. Maeda
  11. Blood v.72 Enzymatic amplification of HTLV-I viral sequence from peripheral blood mononuclear cell and infected tissue Kwok, S.;G. Ehrlich;B. Poieze;R. Kalish;J. J. Shinsky
  12. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli, U.K.
  13. J. Infect. Dis. v.162 Determination of a unique and immunodominant epitope of human T-cell lymphotropic virus type I Lika, J. J.;K. Bui;G. R. Reyes;R. Moechi;S. Z. Wiktor;W. A. Blattner;E. L. Murphy;G. M. Shaw;C. V. Hanson;J. J. Snisky;S. K. Foung
  14. J. Infect. Dis. v.164 Modified western blot assay for confirmation and differentiation of human T-cell lymphotropic virus type I and II Lika, J.J.;P. Santiago;L. Chan;G. R. Reyes;K. P. Samuel;W. A. Blattner;G. M. Shaw;C. V. Hanson;J. J. Sninsky;S. K. Foung
  15. J. Clin. Microbiol. v.28 Development and evalutation of a human T-cell leukemia virus type I serological assay incorporating a recombinant envelope polypeptide Lillehoj, E.P.;S.S. Alexander;C.J.Dubrule;S. Wiktor;R. Andams;C.C.Tai;A. Manns;W.A. Blattner
  16. Blood v.77 Detection of early human T-cell lymphotropic virus type I antibody pattern during seroconversion among transfusion recipients Manns, A.;E. L. Murphy;R. Wilks;G. Haynes;J. P. Figueroa;B. Hanchard;M. Barnett;J. Drummond;D. Waters;M. Cerney;J. R. Seals;S. S. Alexander;H. Lee;W. A. Blattner
  17. Proc. Natl. Acad. Sci. USA v.77 Detection and isolation of type C retrovirus particles from fresh and culture lymphocytes of patient with cutaneous T-cell lymphorma Poisz, B.J.;F.W. Ruscetti;A.F. Gazdar;P.A. Bunn;J.D. Minna;R.D. Gallo
  18. Science v.239 Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Saiki, R. K.;D. H. Gelfand;S. Stoffel;S. J. Scharf;R. Higuchi;G. J. Horn;K. B. Mullis;H. A.Erlich
  19. Science v.226 Diagnostic potential for human malignancies of bacterial produced HTLV-I envelope protein Samuel, K.P.;J.A.Lautenberger;C.L. Jorcyk;S. Josephs;F. Wong-Staal;T.S. Papis
  20. Virology v.132 Sera from adult T-cell leukemia patients reactive with envelope and core polypeptides of adult T-cell leukemia virus Schneider, J.;N. Yamamoto;Y. Hinuma;G. Hunsmann
  21. Blood v.50 Adult T-cell leukemia:Clinical and hematologic features of 16 cases Uchiyama, Y.;J. Yoda;K. Sagawa;K. Takatsuki;H. Uchino
  22. J. Clin. Microbiol. v.33 Enhanced specificity of truncated transmembrane protein for serologic confirmation of human T-cell lymphotropic virus type 1(HTLV-1)and HTLV-2 infections by western blot(immunoblot) assay containing recombinant envelope glycoproteins Varma, M.;D. L. Rudolph;M. Knuchel;W. M. Switzer;K. G. Hanlock;M. Velligan;L. Chan;S. K. H. Foung;R. B. Lal
  23. Proc. Natl. Acad. Sci. USA v.79 Isolation and characterization of retrovirus from cell lines of human adult T-cell leukemia and its implications in the disease Yoshida, M.;I. Miyoshi;Y. Hinuma