Ethanol Production from Lignocellulosic Biomass by Simultaneous Saccharification and Fermentation Employing the Reuse of Yeast and Enzyme

  • KIM, JUN-SUK (Department of Chemical Engineering, Korea University) ;
  • KYUNG-KEUN OH (Department of Industrial Chemistry, Dankook University) ;
  • SEUNG-WOOK KIM (Department of Chemical Engineering, Korea University) ;
  • YONG-SEOB JEONG (Faculty of Biotechnology, Chonbuk National University) ;
  • SUK-IN HONG (Department of Chemical Engineering, Korea University)
  • 발행 : 1999.06.01

초록

Simultaneous saccharification and fermentation (SSF) experiments were carried out with a lignocellulosic biomass. The effects of temperature on enzymatic saccharification and the ethanol fermentation were also investigated. The batch SSF process gave a final ethanol concentration of 10.44 g/l and equivalent glucose yield of 0.55 g/g, which was increased by 67% or higher over the saccharification at 42℃. The optimal operating condition was found to vary in several parameters, such as the transmembrane pressure, permeation rate, and separation coefficient, related to the SSF combined with membrane system (semi-batch system). When the fermentation was operated in a semi-batch mode, the efficiency of the enzymes and yeast lasted three times longer than in a batch mode.

키워드

참고문헌

  1. Appl. Biochem. Biotechnol. v.39/40 Optimization of the simultaneous saccharification and fermentation process using thermotolerant yeasts Ballesteros, I.;J. M. Oliva;M. Ballesteros;J. Carrasco
  2. Biotechnol. Bioeng. v.41 Cell retention culture with an internal filter module Chang, H.N.;W.G.Lee;B.S.Kim
  3. Biotechnol. Lett. v.5 Optimization of process design for continuous ethanol production by Zymmonas mobilis Charley,R.C.;J.E.Fein;B.H.Laverse;H.G.Lawford;G.R.Lawford
  4. Biotechnol. Lett. v.5 Ethanol production in a membrane recycle bioreactor for continuous fermentation of lactose to ethanol Cheryan,M.;M.A. Mehaia
  5. Process Biochem v.19 Ethanol production in a membrane recycle bioreactor Cheryan,M.;M.A.Mehaia
  6. Bioenergy '84 Operating experience from a biostil grain alcohol plant Cook, R.;M. Wallner;L. Garlick
  7. Biotechnol. Bioeng. v.26 Simultaneous saccharification and fermentation (SSF) of lignocellulosics to ethanol under vacuum cycling and step feeding Ghose T. K.;P. K. Roychoury;P. Ghosh
  8. Pure & Appl. Chem. v.59 Measurement of cellulase activities Ghose, T.K.
  9. Appl. Biochem. Biotechnol. v.34/35 Preliminary estimate of the cost of ethanol production for SSF technology Hinman,N.D.;D.J. Schell;C.J. Riley;P.W.Bergeron;P.J.Water
  10. Biotechnol. Bioeng. v.26 The HCH-1 model of enzymatic cellulose hydrolysis Holtzapple,M.T.;H.S.Caram;A.E.Humpery
  11. J. Ferment. Technol. v.66 Ethanol production in simultaneous saccharification and fermentation of cellulose with temperature profiling Huang, S. Y.;J. C. Chen
  12. Kor. J. Biotechnol. Bioeng. v.11 Enzyme deactivation during enzyme recycling with ultrafiltration hollow fiber Kim, J. S.;Y. S. Jeong;S. I. Hong
  13. Biotechnol. Bioeng. v.42 Some factors determining protein aggregation during ultrafiltration Kim, K. J.;V. Chen;A. G. Fan
  14. Biotechnol. Lett. v.9 High yeast concentration in continuous fermentation with cell recycle obtained by tangential microfiltration Lafforgue, C.;J. Malinowski;G. Goma
  15. Biotechnol. Bioeng. v.29 Kinetics of ethanol fermentations in membrane cell recycle fermenters Lee, C. W.;H. N. Chang
  16. J. Kor. Inst. Chem. Eng. v.32 Optimization of pretreatment conditions for enzymatic hydrolysis of lignocellulosic biomass Lee,J.S.;J.P.Lee;J.K.Cho;Y.W.Lee;J.J.Hong;S.C.Park
  17. Science v.251 Fuel ethanol from cellulose biomass Lynd,L.R.;J.H.Cushman;R.J.Nichols;C.E.Wyman
  18. Adv. Chem. Ser. v.95 The production of cellulases Mandels, M.;J. Weber
  19. Analytical Chem. v.31 Use of dinitrosalicylic acid reagent for determination of reducing sugar Miller, G. L.
  20. J. Microbiol. Biotechnol. v.8 Development of cellulose-utilizing recombinant yeast for ethanol production from cellulose hydrolyzate Pack, S. P.;K. M. Cho;H. S. Kang;Y. J. Yoo
  21. Biotechnol. Bioeng. v.41 Study of the enzymatic hydrolysis of cellulose for production of fuel ethanol by the simultaneous sacchairification and fermentation process Philippidis, G. P.;T. K. Smith;C. E. Wyman
  22. J. Inst. Brew. v.76 Measurement of yeast viability Pierce, J. S.
  23. Appl. Biochem. Biotechnol. v.17 Thermotolerant yeast for simultaneous saccharification and fernentation of cellulose to ethanol Spindler, D. D.;C. E. Wyman;A. Mohagheghi;K. Grohmann
  24. Proc. Biocony. Symp. A method for production of alcohol directly from cellulose using cellulase and yeast Takagi, M.;S. Abe;S. Suzuki;G. H. Emert;N. Yata