Journal of Microbiology and Biotechnology

  • 제9권3호
  • /
  • Pages.292-296
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  • 1999
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Direct Purification of Lysozyme from Hen Egg White Using High Density Mixed Mode Adsorbent

  • KIM, WON KYUNG (Bioseparation Process Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • BONG HYUN CHUNG (Bioseparation Process Research Unit, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 1999.06.01
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초록

The high density mixed mode adsorbent known by the trade name of Mimo-AD was used to purify lysozyme directly from the hen egg white (HEW). The homogenized hen egg white was treated with the adsorbent in a stirred vessel for lysozyme adsorption, and then the adsorbent, easily separated from the HEW by sedimentation, was packed into a column. The remaining HEW and contaminant proteins were removed by washing with pH 11 distilled water in an expanded-bed state, and subsequently the elution was performed with pH 12 distilled water in a packed-bed state. By this simple and rapid adsorption, washing, and elution procedure, lysozyme was purified to＞95％ with an overall recovery yield of 66％. This process offers a great potential for industrial application by allowing the extraction of lysozyme while retaining the commercial value of HEW.

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