Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Direct Purification of Lysozyme from Hen Egg White Using High Density Mixed Mode Adsorbent

  • KIM, WON KYUNG (Bioseparation Process Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • BONG HYUN CHUNG (Bioseparation Process Research Unit, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 1999.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The high density mixed mode adsorbent known by the trade name of Mimo-AD was used to purify lysozyme directly from the hen egg white (HEW). The homogenized hen egg white was treated with the adsorbent in a stirred vessel for lysozyme adsorption, and then the adsorbent, easily separated from the HEW by sedimentation, was packed into a column. The remaining HEW and contaminant proteins were removed by washing with pH 11 distilled water in an expanded-bed state, and subsequently the elution was performed with pH 12 distilled water in a packed-bed state. By this simple and rapid adsorption, washing, and elution procedure, lysozyme was purified to＞95％ with an overall recovery yield of 66％. This process offers a great potential for industrial application by allowing the extraction of lysozyme while retaining the commercial value of HEW.

Keywords

References

  1. Nippon Nougeikagaku Kaishi v.46 The preservative effect of egg white lysozyme to non-packed Kamaboko Akashi, A.;A. Ohno
  2. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantitics of protein utilizing the principle of protein-dye binding Bradford, M.
  3. Proc. Roy. Soc. London Ser. B. v.93 On a remarkable bacteriolytic element found in tissues and secretions Fleming, A.
  4. Int. J. Protein Res. v.20 Pigeon egg white lysozyme: Purification, structural and enzymic characterization Gavilanes, J. G.;G. G. Buitrago;A. M. Pozo;R. Perez-Castells;R. Rodriguez
  5. Arch. Biochem. Biophys v.183 Lysozyme in egg white of tortoises and turtle Gayen, S. K.;S. Som;N. K. Sinha;A. Son
  6. Food Addit. Contam. v.9 Lysozyme: Just an additive or a technological aid as well? Giangiacomo,R.;F.Nigro;G.Messina;T.M.Cattaneo
  7. Trends Biotechnol. v.15 Expanded-bed adsorption in industrial bioprocessing: Recent development Hjorth, R.
  8. Bioseparation v.5 Analysis of some operating parameters of novel adsorbents for recovery of proteins in expanded beds Hjorth, R.;S. Kampe;M. Carlsson
  9. J. Biochem. v.107 Purification, amino acid sequence, and some properties of rabbit kidney lysozyme Ito, Y.;H. Yamada;S. Nakamura;T. Imoto
  10. J. Chromatogr. v.510 Rapid protein purification using phenylbutylamine-Eupergit: A novel method for large-scale procedures Kasche, V.;F. Loffler;T. Scholzen;D. M Kramer
  11. J. Biol. Chem. v.245 Turkey egg white lysozyme: Preparation of the crystalline enzyme and investigation of the amino acid sequence LaRue, J. N.;J. C. Spec
  12. Chem. Rev. v.89 Mixed-mode chromatography of nucleic acids Mclaughlin, L. W.
  13. Bioseparation v.2 Resolution and productivity of conalbumin and lysozyme fresh egg-white loaded at very high flow rate on a 250-mm length CM-HVFM column Ming, F.;J. A. Howell
  14. Biotechnol. Tech. v.6 Application of a novel $Productive^{TM}$CM column in egg-white protein separation: Effect of high flow rate on resolution and productivity Ming, F.;J. A. Howell
  15. Biotechnol. Bioeng. v.42 Study on separation of conalbumin and lysozyme from high concentration fresh egg white at high flow rate by a novel ion-exchanger Ming, F.;J. A. Howell
  16. J. Chromatogr. A v.757 Direct purification of lysozyme using continuous counter-current expanded bed adsorption Owen, R. O.;H. A. Chase
  17. Biochimie v.58 Enzymatic properties of a new type of lysozyme isolated from Asterius rubens: Comparison with the Nephthys hombergii(annelid)and hen lysozymes Perin, J.P.;P. Jolles
  18. Biotechol. Prog. v.4 Dual-functional affinity protein purification Russan, R.-C.;B. J. Blair;J. A. Shaeiwiz
  19. J. Biochem. Biophys. Methods v.27 Batch isolation of hen egg white lysozyme with magnetic chitin Safarik. I.;M. Safarikova
  20. J. Chromatogr. v.510 Affinity-repulsion chromatography Teichberg, V. I.
  21. Anal. Biochem. v.139 Purification of human lysozyme from milk and pancreatic juice Wang, C.S.;H. U. Kloer
  22. Biochem. J. v.126 Chromatography of lipopohilic proteins on adsorbents containing mixed hydrophobic and ionic groups Yon, R. J.
  23. Biochem. J. v.151 Protein chromatography on adsorbents with hydrophobic and ionic groups Yon, R.J.;R.J.Simmonds